Disposable microfluidic cartridges,
potentiostat reader devices, systems, kits, and methods to determine the concentration of
analyte reaction products in a patient's specimen, such as: blood,
saliva,
cerebrospinal fluid,
joint fluid or
tears. The microfluidic cartridges are disposable, paper-based strips comprising multiple
assay layers, each layer comprising: a drop zone for receiving a patient specimen; a filter to remove contaminates, and / or isolate
assay reactants; a hydrophilic
microfluidic channel to direct movement of the specimen down the
cartridge; a centered
reaction chamber comprising impregnated enzymes and reagents to chemically react with the filtered specimen to produce an
analyte; and a
detector mechanism. Multiple analytes can be produced in parallel (e.g. one per layer), or sequentially along one layer. The
detector mechanism utilizes electro-analytic methods to facilitate the reader device in quantifying each
analyte, and each analyte concentration is wirelessly transmitted to an electronic computing device for storage in a patient's
medical record.