The invention provides a calculus removal medicine composition and a preparation method thereof, and relates to the technical field of medicine. The calculus removal medicine composition is mainly prepared from steatite, rhizoma athyrii, endothelium corneum gigeriae galli, herba lysimachiae, herba hedyotis diffusae, lygodium japonicum, plantain herb, rhizoma imperatae and comanet grape roots, wherein the rhizoma imperatae and the comanet grape roots are used as monarch medicine, and are used for enabling the urination of a user to be smooth by eliminating inflammation, excreting gall bladder,removing external heat and nourishing yin; the calculus removal effects are achieved; the plantain herb, the rhizoma athyrii and the endothelium corneum gigeriae galli are used as minister medicine and are used for assisting the monarch medicine to achieve the calculus removal effects by promoting spleen operation, promoting digestion, relieving swelling and pain, inducing diuresis to alleviate water retention, diminishing inflammation, removing calculus and regulating electrolyte balance, so that the calculus removal is smooth, and the calculus removal effect taking speed is higher. The steatite, the herba lysimachiae and the lygodium japonicum are used as adjuvant medicine; the calculus removal effect can be enhanced; the disease pain of the patient can be eliminated through alleviatingwater retention, relieving pain, diminishing inflammation and relieving swelling. The herba hedyotis is used as guide medicine, and has the effects of conditioning various medicine, regulating yin andyang, alleviating water retention and eliminating calculus.