The invention relates to a rock-soil body slope water motion simulation device containing a root stone structure and an experiment method, the device mainly comprises a box body, the top surface of the box body is a slope, two end surfaces, a bottom plate and one side surface of the box body are cement outer walls, the two end surfaces are respectively positioned at the upper end and the lower end of the slope, and the other side surface is a glass observation wall; the box body is sequentially segmented from the highest end to the lowest end, and the depth of each segment of area is gradually increased in sequence; a water outlet is reserved at the bottom of the lowest part in each section of area, and an effluent liquid collector is arranged at the water outlet; a backfill soil layer is arranged in each section of area; a soil moisture probe and a soil solution collector are arranged in the backfill soil layer; ground surface vegetation is planted on the surface of the backfill soil layer, and a high-definition camera is arranged outside the glass observation wall. According to the invention, economic, feasible, scientific and reasonable field large hydrological physical models and test methods and steps are provided for research on root stone-containing soil moisture movement, especially preferential flow, and experimental data can provide reference for mountain hydrological process research and mountain ecological hydrological models.