The invention relates to the field of object three-dimensional positioning methods, and particularly relates to a pixel-level target positioning method based on laser and monocular vision fusion. Themethod comprises the steps of firstly, mounting a camera and a laser ranging module; constructing a coordinate system, calibrating the distance measurement relative positions of the monocular camera and the laser ranging module to obtain calibration parameters, acquiring an environment image through the monocular camera, and then carrying out preprocessing, determining pixel coordinates of a to-be-positioned target; then driving an actuating mechanism through e PID feedback control to drive the laser ranging module to rotate to acquire data and perform calculation; and finally, driving the laser ranging module to accurately shoot a ranging light spot to a target position, acquiring data, and achieving target positioning through calculation. The method is low in computing resource consumption, capable of guaranteeing real-time performance under the condition of limited computing power of the mobile terminal, high in precision, wide in range and capable of improving the operation range of the intelligent autonomous operation equipment, has a certain cost advantage, reduces the use threshold, and is beneficial to technical popularization and application.