The invention relates to a
lung tumor three-dimensional culture method and a culture medium, and discloses a method for constructing a three-dimensional
culture model in a manner of infusion and
oxygen therapy from a shell structure to a culture structure, simulating a complex microenvironment of
lung tissues, providing a stable
culture environment and improving the culture efficiency. The invention relates to a culture method and a culture medium for realizing three-dimensional culture of
lung tumor cells. The culture medium is characterized by comprising a shell structure and a culture structure, the culture structure is arranged in the shell structure, the shell structure is integrated with a gas-liquid conveying
system, the culture structure is a bionic model simulating the shapes of left and right lung lobes, three-stage or four-stage
branch air passages (similar to
bronchus and pulmonary alveoli) are arranged in the culture structure so as to realize uniform distribution of
oxygen, different types of cells are inoculated in the culture structure layer by layer, and the culture structure is integrated with the gas-liquid conveying
system. Functional interaction of different
cell types is ensured, culture conditions are provided in the mode that infusion and
oxygen therapy are conducted on the culture structure from the shell structure, the
lung tumor microenvironment is simulated, and culture of
lung tumor cells is achieved.