The invention discloses a
molecular imaging method based on a high-stability
capillary electrophoresis separation analysis technology, and relates to multiple fields of
analytical chemistry, life science, clinical
medicine,
medicine development, material science and the like. The
molecular imaging method comprises the steps of sample pretreatment, high-stability
capillary electrophoresis sample introduction, separation and analysis and
molecular distribution image drawing. The sample pretreatment adopts a grid knife method, and comprises the steps of uniform segmentation, in-situ extraction, enrichment, derivation and the like. According to the method, the strong separation capability and abundant separation
modes of
capillary electrophoresis are utilized, imaging elements are abundant, and neutral molecules difficult to distinguish by
mass spectrometry and components with the same
mass-to-charge ratio can be imaged. The
batch processing capacity of capillary array
electrophoresis can accelerate the
separation process and shorten the
imaging analysis time. By means of the high-stability capillary
electrophoresis technology, the peak position of the separated component can be stabilized, identification and positioning of the component are facilitated, and then a
spatial distribution image of the stable peak component is constructed.