The invention discloses a fluorine-containing heat transfer fluid. The fluid comprises any one or more structures as described in a formula (I) and/or any one or more structures as described in a formula (II). In the formulas, R1 is H, F or an alkoxy group, R2 is H, F, Cl or an alkoxy group, R3 and R4 are F or alkoxy groups, R5 is H, F or an alkoxy group, and the alkoxy group is -O-C<x>F<y>H<2x+1-y>; and x is a natural number, y is a non-negative integer, and n is 1, 2 or 3. The invention also discloses a preparation method of the fluorine-containing heat transfer fluid. The preparation methodcomprises the step of carrying out a nucleophilic substitution reaction on halogenated cycloolefin and alcohol to prepare the fluorine-containing heat transfer fluid as shown in the formula (I). Thefluorine-containing heat transfer fluid provided by the invention has the advantages of high dielectric strength, low toxicity, low global warming potential, good heat transfer performance, high boiling point, simple synthetic route and high synthetic efficiency, and is suitable for being used as a heat transfer fluid.