The invention discloses a medium-manganese, high-hardness and high-tenacity wear-resisting ball. The medium-manganese, high-hardness and high-tenacity wear-resisting ball comprises, by weight, 3.3%-3.7% of C, 5.5%-6.5% of Mn, less than 0.04% of S, less than 0.1% of P, 3.6%-4.6% of Si, 14.83%-16.83% of Cr, 0.012%-0.018% of Mo, 0.05%-0.09% of V, 0.01%-0.02% of W, 0.02%-0.06% of Ti, 0.01%-0.03% of Bi, 0.02%-0.045% of rare earth, 0.04%-0.055% of Mg, and the balance Fe and inevitable impurities. The invention further provides a preparation method of the medium-manganese, high-hardness and high-tenacity wear-resisting ball. According to the medium-manganese wear-resisting ball prepared through the method, by reasonably selecting the components, the wear resistance and corrosion resistance of the wear-resisting ball are improved, and the service life of the wear-resisting ball is prolonged.