An organ
perfusion apparatus and method monitor, sustain and / or restore viability of organs and preserve organs for storage and / or transport. Other apparatus include an organ
transporter, an organ cassette and an organ diagnostic device. The method includes perfusing the organ at hypothermic and / or normothermic temperatures, preferably after hypothermic organ flushing for organ transport and / or storage. The method can be practiced with prior or subsequent static or
perfusion hypothermic
exposure of the organ. Organ viability is restored by restoring
high energy nucleotide (e.g., ATP) levels by perfusing the organ with a medical fluid, such as an oxygenated cross-linked
hemoglobin-based
bicarbonate medical fluid, at normothermic temperatures. In
perfusion, organ perfusion pressure is preferably controlled in response to a sensor disposed in an end of tubing placed in the organ, by a pneumatically pressurized medical fluid reservoir, providing perfusion pressure
fine tuning, overpressurization prevention and emergency flow
cut-off. In the hypothermic mode, the organ is perfused with a medical fluid, preferably a simple crystalloid solution containing antioxidants, intermittently or in slow
continuous flow. The medical fluid may be fed into the organ from an intermediary tank having a low pressure head to avoid organ overpressurization. Preventing overpressurization prevents or reduces damage to vascular endothelial lining and to organ tissue in general. Viability of the organ may be automatically monitored, preferably by monitoring characteristics of the medical fluid perfusate. The perfusion process can be automatically controlled using a control program.