The invention relates to the field of medical equipment, in particular to a guide wire storage device. The guide wire storage device comprises a stationary shaft, a supporting mechanism and a windingmechanism, the supporting mechanism is fixedly connected with the two ends of the stationary shaft, wherein the winding mechanism comprises a winding roller, a locking block, a handle and a buffer block, the winding roller is coaxially and fixedly arranged on the stationary shaft, the outer surface of the winding roller is provided with a helical guide groove, the buffer block is fixedly arrangedin the guide groove, and the buffer block is internally provided with a through hole allowing a guide wire to run through; the two opposite side walls in the guide groove are provided with sliding grooves, the two sides of the locking block are fixedly provided with limiting blocks sliding in the sliding grooves, and the locking block is in sliding contact with the inner wall of the guide groove;the locking block is internally provided with a mounting hole clamping a guide wire, and the mounting hole can be opposite to the through hole; the side, away from a transfer roller, of the locking block is provided with a threaded hole communicated with the mounting hole, the handle is in threaded connection with the threaded hole, and the end, close to the locking block, of the handle can abut against the inner surface of the mounting hole. The guide wire storage device can prevent the inner wall of a catheter from being damaged.