The invention discloses an 
in vitro multi-channel micro-current stimulator used in the field of an artificial 
nerve repair technology, which comprises a pulse 
signal generator, 
voltage-current conversion circuits, and a multi-channel connector, wherein the output end of the pulse 
signal generator is connected with input ends of the 
voltage-current conversion circuits, output ends of the 
voltage-current conversion circuits are all connected with the multi-channel connector, the pulse 
signal generator generates a single-phase pulse square-wave voltage signal and outputs the signal to the voltage-current conversion circuits, and the voltage-current conversion circuits convert the single-phase pulse square-wave voltage signal into a single-phase 
pulse current signal; and when the voltage-current conversion circuits convert the single-phase pulse square-wave voltage signal into the single-phase 
pulse current signal, the frequency and the pulse width of pulse are adjusted through the pulse 
signal generator, and the pulse amplitude is adjusted through a variable 
resistor Rref. The frequency, the pulse width, and the amplitude output by the 
in vitro multi-channel micro-current stimulator have larger adjustment range, and a device is easy to manufacture and has lower cost.