This invention relates to the field of bioengineering technology, specifically to an endogenous
signal peptide element fused with the MF-αpro region and its applications. The endogenous
signal peptide element is composed of an endogenous
signal peptide from
Pichia pastoris with the C-terminus fused with MF-αpro or with amino acids 38 to 51 of the MF-αpro region detached; the
amino acid sequence of the MF-αpro region is shown in SEQ ID NO:8; the
amino acid sequence of the MF-αpro region with detached amino acids 38 to 51 is shown in SEQ ID NO:10. This invention provides a complete solution from rational screening to
functional verification to element optimization. The resulting highly efficient, patent-free
signal peptide element is suitable for the production of
industrial enzymes, pharmaceutical proteins, and diagnostic reagents.