The invention discloses a multi-factor width parameter nanofluid heat transfer characteristic experimental system and an experimental method. The system comprises three parts, namely an experimental loop, a pressure control loop and a cooling loop, wherein the experimental loop is composed of an ultrasonic oscillator, a circulating main pump, a heat regenerator, a mass flow meter, a preheating section, an experimental section, a data acquisition system and an electric heating system; the pressure control loop is composed of a high-pressure nitrogen and gas-liquid header and a safety valve; andthe cooling loop is composed of a cooling tower, a circulating pump and a cooling jacket. According to the experimental system, single-phase heat convection, flow boiling heat exchange and critical heat flux experimental researches under multi-factor and width parameter conditions can be performed aiming at nanofluid of different components under the condition that the stability of the nanofluidis maintained. The invention further provides an experimental method. The stability of the nanofluid is ensured, the influence of a dispersing agent or other additives is avoided, and the influence factors and mechanisms of the single-phase heat convection, flow boiling heat exchange and critical heat flux of the nanofluid can be conveniently researched.