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57 results about "Tonsillar Tissue" patented technology

Tonsils are collections of lymphoid tissue facing into the aerodigestive tract. The set of lymphatic tissue known as Waldeyer's tonsillar ring includes the adenoid tonsil, two tubal tonsils, two palatine tonsils, and the lingual tonsil.

Electrosurgical system and method

InactiveUS7001380B2Lower impedanceMinimizing char formationCannulasDiagnosticsBenign conditionEnlarged tonsils
A method is disclosed for treating benign conditions, such as enlarged tonsils and / or adenoids located in a patient's throat or nasopharynx, or soft tissue lesions located in a patient's oropharynx or larynx. According to the method, a space containing the patient's nasopharynx, oropharynx or pharynx and larynx is isolated from the patient's trachea and lungs using an inflatable cuff tracheostomy tube or nasotracheal tube inserted in the patient's trachea. The cuff is inflated to occlude the trachea. The patient is placed in a supine position, whereupon at least a portion of the space containing the nasopharynx and / or oropharynx and larynx is filled with saline. An endoscope is then inserted into the space to view the operative site in which the tonsils or tissue lesion are to be treated. An electrosurgical instrument having an active tissue treatment electrode and a return electrode connected to an electrosurgical generator is then inserted into the space, either along side the endoscope or through the endoscope's working channel. The generator is then operated to apply a radio frequency voltage between the active and return electrodes of the electrosurgical instrument, whereby a conduction path is formed between the active and return electrodes, at least partially through the saline, whereupon the active electrode is manipulated to debulk or otherwise treat the soft tissue lesion or enlarged tonsils and / or adenoids.
Owner:GYRUS MEDICAL LTD

Control of halitosis-generating and other microorganisms in the non-dental upper respiratory tract

Disclosed are safe, simple and effective broad-spectrum treatments for halitosis and other microbial infections of the nondental upper respiratory tract useful to treat bacterial and other microorganism species, including anaerobic bacteria. Electromagnetic radiative energy including visible, and optionally, thermal, RF and / or microwave wavelengths, is topically applied to internal surfaces of the upper respiratory tract to destroy or incapacitate superficial microorganisms without the use of antibiotics. One useful apparatus is a handheld energy applicator having a light output head suitable for treating the back of the tongue and the tonsils and which may be interchangeably provided with extensions to reach the sinuses. The energy applicator can be supported and guided by a mounting device held between the subject's teeth, if desired. Useful embodiments of the invention include preparative treatment of the target surfaces with a photosensitizing agent such as an oxidizing agent or a complementary stain. Optionally a pre-treament procedure may be employed to remove detritus and microfloral overgrowths that may mask more deeply resident target microorganisms. Novel treatments include treatment of halitosis by destruction of bacterial species associated with halitosis, such as Atopobium parvulum, by application of non-ionizing radiative energy to the tonsils and the back of the tongue. Another embodiment comprises a candy bar incorporating a halitosis treatment lamp disposed within the candy.
Owner:VALENT MEDICAL INC

Electrosurgical system and method

InactiveUS20050090819A1Lower impedanceChar formation undesirableCannulasDiagnosticsBenign conditionNose
A method is disclosed for treating benign conditions, such as enlarged tonsils and / or adenoids located in a patient's throat or nasopharynx, or soft tissue lesions located in a patient's oropharynx or larynx. According to the method, a space containing the patient's nasopharynx, oropharynx or pharynx and larynx is isolated from the patient's trachea and lungs using an inflatable cuff tracheostomy tube or nasotracheal tube inserted in the patient's trachea. The cuff is inflated to occlude the trachea. The patient is placed in a supine position, whereupon at least a portion of the space containing the nasopharynx and / or oropharynx and larynx is filled with saline. An endoscope is then inserted into the space to view the operative site in which the tonsils or tissue lesion are to be treated. An electrosurgical instrument having an active tissue treatment electrode and a return electrode connected to an electrosurgical generator is then inserted into the space, either along side the endoscope or through the endoscope's working channel. The generator is then operated to apply a radio frequency voltage between the active and return electrodes of the electrosurgical instrument, whereby a conduction path is formed between the active and return electrodes, at least partially through the saline, whereupon the active electrode is manipulated to debulk or otherwise treat the soft tissue lesion or enlarged tonsils and / or adenoids.
Owner:GYRUS MEDICAL LTD

Control of halitosis-generating and other microorganisms in the non-dental upper respiratory tract

InactiveUS20100076526A1Effective controlReduces gram negative bacterial burdenElectrotherapyLight therapyNon-ionizing radiationAnaerobic bacteria
Disclosed are safe, simple and effective broad-spectrum treatments for halitosis and other microbial infections of the nondental upper respiratory tract useful to treat bacterial and other microorganism species, including anaerobic bacteria. Electromagnetic radiative energy including visible, and optionally, thermal, RF and / or microwave wavelengths, is topically applied to internal surfaces of the upper respiratory tract to destroy or incapacitate superficial microorganisms without the use of antibiotics. One useful apparatus is a handheld energy applicator having a light output head suitable for treating the back of the tongue and the tonsils and which may be interchangeably provided with extensions to reach the sinuses. The energy applicator can be supported and guided by a mounting device held between the subject's teeth, if desired. Useful embodiments of the invention include preparative treatment of the target surfaces with a photosensitizing agent such as an oxidizing agent or a complementary stain. Optionally a pre-treatment procedure may be employed to remove detritus and microfloral overgrowths that may mask more deeply resident target microorganisms. Novel treatments include treatment of halitosis by destruction of bacterial species associated with halitosis, such as Atopobium parvulum, by application of non-ionizing radiative energy to the tonsils and the back of the tongue. Another embodiment comprises a candy bar incorporating a halitosis treatment lamp disposed within the candy.
Owner:KRESPI YOSEF +1

Devices and methods for treating pain associated with tonsillectomies

Described here are devices and methods for treating one or more conditions or symptoms associated with a tonsil procedure. In some variations, a drug-releasing device may be at least partially delivered to one or more tonsillar tissues before, during, or after a tonsil procedure. In some variations, the drug-releasing device may be configured to be biodegradable. In other variations, the drug-releasing device may comprise one or more hemostatic materials or one or more adhesives. The drug-releasing device may be configured to release one or more drugs or agents, such as, for example, one or more analgesics, local anesthetics, vasoconstrictors, antibiotics, combinations thereof and the like.
Owner:INTERSECT ENT INC

Film forming polymeric sealant for medical use

The invention provides a polymeric film-forming medical sealant. The medical sealant is useful for application to the tonsils and adenoids, wherein the sealant performs at least one of the following functions, a) inhibit the colonization of bacteria, b) inhibit the binding of bacteria to tissue, c) reduction of tissue morbidity, d) hemostasis, e) coating and protection of tissue during healing. e) promotion of healing, and f) reduction of pain.
Owner:MEDTRONIC XOMED INC

Recombination of human soluble TRAIL protein, the preparing method and the application in preparing antineoplastic medicine

The present invention discloses recombinant soluble human tumore necrosis factor related death inducing ligand and its preparation process and application in preparing antitumor medicine. The present invention adopts human tonsil tissue mRNA as template to amplify TRAIL coding sequence, constitute engineering colibacillus for expressing rhsTRAIL and engineering saccharomycete, establish the rhsTRAIL engineering colibacillus fermentation, occlusion body washing and destination protein purification process and the engineering saccharomycete and destination protein purification process separately, and obtain pure rhsTRAIL product, which has molecular weight of 19.6-24 KD and exists in both monomer and dimmer. The rhsTRAIL can induce death of several kinds of cancer cells outside body, inhibit amplification of liver cancer cell in tumor loading mouse obviously and kill tumor cell selectively, so that it may be used in preparing effective antitumor medicine.
Owner:李宏

Beveled tonsil suction cautery dissector

A suction cautery dissector comprising a handle assembly, a tubing assembly having a suction channel formed therein, the tubing assembly comprising a first portion connected to the handle assembly and a distal end, and a tip assembly at the distal end of the insulated tubing assembly, the tip assembly comprising a substantially co-planar, enclosed cautery surface with a beveled leading edge sharpened for dissecting tissue. The cautery surface provides an opening formed therein that communicates with the suction channel in the insulated tubing assembly and being operably connected to an electrical source. The tip assembly comprises a tip wall terminating at the cautery surface and wherein the tubing assembly comprises an insulating layer covering the suction channel from the first portion to the distal end, terminating at a minimum predetermined distance along the tip wall from the cautery surface around the circumference of the tip assembly to enable the tip assembly to make surface contact around the entire tip wall.
Owner:OHALLORAN LAURENCE R

Tonsillith removing device

A tonsillith removing device for use in removing tonsillith and other undesired matter from a user's tonsil crypts is described. The device comprises a suction channel element and a suction mechanism unit. The suction force is provided by an electric fan installed in the suction mechanism unit housing. The device has a retention feature for retaining the sucked tonsillith and undesired matter. The suction channel element can be easily detached from the suction mechanism unit and the retained tonsillith and undesired matter can be cleaned.
Owner:PAN HONGJUN

Tonsil Forceps

InactiveUS20120203272A1Surgical forcepsTonsil forcepsForceps
A tonsil forceps having first and second intersecting and pivoting arm members, releasable locking members for securing the forceps in multiple positions and grasping members disposed on said arm members. In at least one of the locked positions, the tines on the grasping members do not cross. The grasping members have a concave interior surface, inwardly facing blunt interior projection members, flush lateral projection members and angled lateral projection members. The projecting members are non-contacting with each other even in the maximum closed forceps position.
Owner:WOHL DANIEL L

Photothermal treatment of soft tissues

ActiveUS8685010B2Delayed tissue involutionCatheterDiagnostic recording/measuringHeat treatedSoft tissue
An apparatus and a method are provided for the treatment of soft tissue. The apparatus includes a light source and at least two optical assemblies. Each of the optical assemblies includes at least one optical element and a light-transmitting contact surface configured to transmit a substantially uniform distribution of light therethrough. The apparatus further includes at least two optical transmission devices each disposed between the light source and a corresponding one of the at least two optical assemblies. The apparatus also includes a handpiece to which the at least two optical assemblies are attached, and which is adapted to bring the light-transmitting contact surfaces of the at least two optical assemblies in contact with soft tissue disposed therebetween. The method includes compressing and substantially flattening portions of the tonsil tissue between light-transmitting contact surfaces of two opposed light emitting optical assemblies, introducing light into an optical element of each of the optical assemblies; and irradiating the tonsil tissue by directing the light from the optical element through the light-transmitting contact surfaces to the tonsil tissue in a substantially uniform light distribution.
Owner:GRADIANT RES

Traditional Chinese medicine mouth wash for cleaning mouth cavity and preparation method thereof

InactiveCN105769718AGood antibacterial effectClearing away heat, cooling blood and detoxificationAntibacterial agentsCosmetic preparationsMouth washThroat
The invention discloses a traditional Chinese medicine mouth wash for cleaning the mouth cavity. The traditional Chinese medicine mouth wash is prepared from, by weight, 40-60 parts of radix sophorae flavescentis, 80-110 parts of radix isatidis, 20-40 parts of honeysuckle flowers, 30-50 parts of rhizoma atractylodis, 30-40 parts of herba verbenae, 30-40 parts of Chinese magnoliavine fruits and 15-25 parts of mint. The traditional Chinese medicine mouth wash has the effects of removing heat, cooling blood and eliminating toxins, and resisting bacteria, diminishing inflammation and easing pain, is suitable for daily cleaning of the mouth cavity, is also suitable for bacterial infection or viral infection caused by bacterial dysfunction of the mouth cavity or the throat or the tonsil or other parts, swelling and aching of gums, dental ulcers, ozostomia and the like, can make the mouth cavity fragrant, clean, fresh, comfortable and natural and can be used for a long term.
Owner:山西振东健康护理科技股份有限公司

Pediatric tonsil grasper

The invention relates to a pediatric tonsil grasper and belongs to the technical field of tonsil grasper structures. The pediatric tonsil grasper consists of two grasper holders connected into a whole through a clamp nail, wherein each grasper holder comprises an annular grasper handle, a grasper rod and an arc-shaped grasper jaw, an oval grasper head is arranged at the top end of the grasper jaw, the oval grasper head is perpendicular to the axial direction of the grasper jaw, and blunt teeth are arranged on the grasper head; the space between each two adjacent teeth is 1.5 times that of an ordinary grasper; the grasper rod is longer than the grasper jaw; buckling connection between the grasper handles are achieved through a connection rod. According to the pediatric tonsil grasper, by means of the arc-shaped grasper jaw, shielding of an operation handler's view can be effectively avoided, difficulties of an operation are reduced, and a success rate of the operation is increased; meanwhile, by means of the special oval arrangement of the grasper head and the arrangement of the blunt teeth with the large space on the grasper head, the operation is facilitated, surrounding physiological tissue are not prone to be badly grasped, pain of patients is reduced, and success of the operation is facilitated.
Owner:张天振

Tonsil extirpating device

InactiveCN105796157AThe extirpation went wellReduction of surgical excision timeSurgeryMedical devicesEngineeringTube diameter
The invention discloses a tonsil extirpating device. The tonsil extirpating device comprises a sleeved flat tube, a viewing tube and an extirpating mechanism, wherein the sleeved flat tube is used for accommodating a tonsil head to be extirpated; the extirpating mechanism is connected with the sleeved flat tube and the viewing tube; the sleeved flat tube is equal to the viewing tube in tube diameter; the extirpating mechanism comprises an extirpating bracket which is connected with the sleeved flat tube and the viewing tube, and an extirpating tool arranged on the extirpating bracket; the extirpating bracket comprises a top plate, a left side plate, a bottom plate and a right side plate which are connected in sequence; the top plate is provided with a top bump I and a top bump II which is positioned beside the top bump I; both the top bump I and the top bump II are fixedly connected with the top plate; a top gap through which the tool can pass is reserved between the top bump I and the top bump II; the top gap is equal to the extirpating tool in width; the lower surfaces of the top bump I and the top bump II are cambered surfaces; the cambered surface of the top bump I is fixedly connected with the sleeved flat tube; the cambered surface of the top bump II is fixedly connected with the viewing tube. The tonsil extirpating device can be used rapidly, and the probability of bacterial infection is lowered.
Owner:邹燕燕

Method for detecting presumed iga nephropathy and method for screening iga nephropathy patients

InactiveUS20120034611A1Improve accuracySimple and quick manner without physical burden on the patientSugar derivativesMicrobiological testing/measurementBacteroidesBacilli
Based on detection of Treponema bacteria or Campylobacter bacteria present in a sample derived from the tonsil of a subject who has a positive result on at least one of a urinary protein test and a urinary occult blood test, or of a patient diagnosed with possible IgA nephropathy, presumed IgA nephropathy can be detected with a high accuracy, in a simple and quick manner without physical burden on subjects. Also, an IgA nephropathy patient for whom tonsillectomy is effective in therapy of IgA nephropathy can be selected with a high accuracy, in a simple and quick manner without physical burden on subjects.
Owner:OSAKA UNIV

Topical pathogenic-tissue-destroying liquid

The invention relates to a method of producing a pharmaceutical composition, consisting in mixing nitric acid in composition with mucin and ptyalin enzymes which are used as absorbers. Together, said components produce a topical aqueous solution which destroys pathogenic tissue, by coagulating same, in order to treat a patient with warts, verrucas, skin cancer, tongue cancer, throat cancer, ulcerated tonsils, gingivitis, streptococcus or staphylococcus in the throat, uterine cervix cancer or fungus. The inventive composition can also be applied to any part of the skin or mucous membranes. Said composition is administered topically (externally) in one, two, three or four applications depending on the severity of the infection or wound. More specifically, the invention relates to a pharmaceutical composition comprising an effective quantity of nitric acid, mucin and ptyalin and distilled water which is used as an acceptable pharmaceutical vehicle or carrier. The composition is selective given that it strangulates the pathogenic tissue until said tissue is destroyed, leaving the healthy tissue intact apart from a slight mark which is caused by the effects of the nitric acid.
Owner:SUAREZ MENDOZA RAMON

Tonsil inflammation medicine application device

The invention relates to a medicine application device, in particular to a tonsil inflammation medicine application device. The technical purpose is to provide the tonsilinflammation medicine application device capable of opening patient mouths to avoid influence on the application of medicines caused by closing of the mouths, and proving more convenience formedical staff to apply the medicines onthe tonsil. According to the technical scheme,the tonsil inflammation medicine application devicecomprises a bottom plate, an n-shaped plate, a guide rod, a guide sleeve, a supporting rod, a hollow tube, a grip rod, a first spring, a movable block, a pull plate,a curved plate, a sphere, a perforated plate, a clamping bar and the like; an opening is formed in the left side of the bottom plate,then-shaped plate which can open the patientmouths is hinged between the left sides of the front and rear two side surfaces of the bottom plate, and the right side of the bottom of the n-shaped plate isfixed tothe guide rod. The device swings to the right by pulling the supporting rod, so that the n-shaped plate swings upward to support the patientmouths, a patient does not need to open his mouth, which avoids the mouth closing affecting the medicine application,through the action of the perforated plate, and more convenience is provided for the medical staff to apply the medicines to the tonsilwithout using a cotton swab to avoid puncturing the patient mouths.
Owner:万秀芳

Sampling method and detection method of detecting common vertical transmission pathogen of newborn piglet

The invention provides a sampling method and a detection method of detecting a common vertical transmission pathogen of a newborn piglet. The sampling method comprises the step of sampling the vertical transmission pathogen via afterbirth, cord blood, a tonsil, blood, a nose swab, a throat swab and an anus swab. During detection of a virus, one or more detection samples are processed; viral nucleic acid is extracted; a primer is designed for PCR (polymerase chain reaction) or RT-PCR (reverse transcription-polymerase chain reaction) amplification; agarose gel electrophoresis detection is performed on an amplification product. The sampling method and the detection method can detect the common vertical transmission pathogen of the piglet relatively comprehensively, are simple to operate, short in required lime and high in pertinency, avoid the disadvantages of complex operation, long time consumption, low detection rate and the like of the traditional sampling and detection methods, and facilitate disease prevention and control and purification of the newborn piglet.
Owner:SHANGHAI CHUANG HONG BIOTECH

Tonsillar suction dissector

The various embodiments of the present invention provide a tonsillar suction dissector for performing the functions of dissecting a tonsil and suctioning blood simultaneously. The tonsillar suction dissector includes a handle, a suction tube connected to one end of the handle, a proximal part coupled to a proximal end, a serrated distal end connected to the proximal part through the suction tube, a rolling part interconnected to the proximal part and the proximal end, and a tip assembly. The opening suctioning channels placed adjacent to the serrated distal end provide an opening to communicate with the handle and allows the suction tube to suction to clear a dissection surface thereby performing the functions of dissecting a tonsil and suctioning the dissection surface simultaneously.
Owner:FARHADI MOHAMAD +1

Method for labelling human hepatic tissue capillary bile duct with CD25/IL-2Ra antibody

A method that using CD25 / IL-2Ra antibody marks capillary bile duct of human liver organize, it belongs to histiocyte coloration expression technology field. This invention uses human liver puncture biopsy as specimen, the specimen is repaired by antigen, uses CD25 / IL-2Ra as the first antibody, color and flaking by immunity grouping or immunity reflected color, ensure its specificity by interdiction test, and ensure the marked capillary bile duct of human liver organize. Using two CD25 / IL-2Ra in different clone number as the first antibody, immunity grouping liver capillary bile duct is brow yellow bar structure, the expression effect are good, or the interdiction test ensure that the liver capillary bile duct expresses the specificity of CD25 / IL-2Ra by the result that the immunity reflected color liver capillary bile duct has green fluorescence. We set antitheses between masculine antitheses(tonsils) and CD4,CD8, the result indicates that the color of immunity grouping is in focus, the orientation is correct, the same organization use CD25 / IL-2Ra and CEA as antitheses separately, it proves that the CD25 / IL-2Ra is more specific than CEA, its color effect is well than CEA, it is benefit for the live capillary bile duct distributing and morphologic observation.
Owner:无锡市传染病医院

Dynamic diagnosis and treatment simulation device for human pharynx and larynx

PendingCN109887366AIncrease diagnosis and treatment experienceCosmonautic condition simulationsEducational modelsHuman bodyPalate muscle
A dynamic diagnosis and treatment simulation device for human body pharynx and throat comprises a skull model designed according to the structure and the position of a human body, a soft palate model,a tongue model, a tonsil model and a throat model, wherein the soft palate model, the tongue model, the tonsil model and the throat model are located in an inner cavity of the skull. The skull modeluses the rear wall of the coronal pharyngeal cavity-throat model as a boundary and is provided with a front part and a rear part which are connected through a locking device, the front part is a basepart to which the soft palate model, the tongue model, the tonsil model and the throat model are attached, the rear part is provided with a linkage device, and the linkage device is electrically connected with the soft palate model, the tongue model, the tonsil model and the throat model. The dynamic diagnosis and treatment simulation device can be used for assisting in learning of laryngopharyngeal diagnosis and treatment technologies, including indirect laryngoscope, hard lens, foreign body removal and the like.
Owner:XIANGYA HOSPITAL CENT SOUTH UNIV

Method for culturing differentiation-promoting and -sustaining spheroid form of tonsil-derived stem cells

The present invention relates to: a method for producing a spheroid form of tonsil-derived stem cells, the method enhancing growth and differentiation efficiency of tonsil-derived stem cells; and a method for producing a spheroid form of parathyroid hormones from the same. The method of the present invention for producing a spheroid form of tonsil-derived stem cells cultures the tonsil-derived stem cells in the form of a spheroid to thereby enhance the proliferation rate of the stem cells per se and remarkably increase differentiation potency into parathyroid cells. Thereby, the method has a remarkable effect on the growth and differentiation of the tonsil-derived stem cells into parathyroid cells, etc.
Owner:EWHA UNIV IND COLLABORATION FOUND +1

Compound ilexlutifolia thumb preparation for treating acute tonsil inflammation

The invention provides a compound broadleaf holly leaf preparation for curing acute tonsillitis, which comprises the following components: great burdock achene, balloon flower, liquorice, flos lonicerae, weeping forsythia, subprostrate sophora, tinospora root, reed root, peppermint, mongolian snakegourd root, lobster sauce, herba schizonepetae, mulberry leaf, flos chrysanthemi, spica prunellae, green tea, broadleaf holly leaf, etc. The preparation can cure the diseases such as acute tonsillitis, tonsil swelling as well as fever and pain caused by acute tonsillitis. The preparation has the advantages and the effects that the total effective rate of the clinical cure reaches 100%, and fills the blank of the subject field of curing the diseases, such as acute tonsillitis and the complicating diseases thereof, by adopting the compound broadleaf holly leaf preparation.
Owner:李运乃

Atmosphere regulating cream special for children

InactiveCN101176761ARelieve painSolve the fear of injectionsAerosol deliveryDigestive systemNostrilPoultice
The invention discloses a paediatric Qi-regulating ointment, with Chinese herbal medicine hemp seed and Evodia Rutaecarpa as the raw material. After being grinded and sifted out, the materials are modulated into plaster with vinegar or honey, which can stick to the skin above the Yongquan Point or the Qihai Point to produce a better therapeutic effect on a plurality of diseases, such as the paediatric oral ulcer, oral inflammation, and acute inflammation of tonsil and nostril. The ointment is for external use and has the advantages of security, convenience and definite therapeutic effect. The ointment is confirmed to have evident efficacy without toxin and adverse effect in clinical use.
Owner:王玉林

Laryngeal image processing method

The invention relates to a laryngeal image processing method, which comprises the following steps of: segmenting a laryngeal image by adopting a curve evolution method, wherein the segmented image comprises a tonsil, a palate sag around the tonsil and a soft palate part above the tonsil; Analyzing the segmented complete laryngeal image, extracting laryngeal image characteristics including colors,shapes and laryngeal qualities, carrying out color extraction analysis and comparison on the color characteristics through a fixed RGB space, and regarding the throat image characteristics as inflammation if the color is slightly red and greater than a normal color value. the shape judgment is to judge whether the tonsil and the surrounding palate sag have too large or too small glandular hyperplasia or not and whether the shape meets the standard or not.
Owner:TIANJIN UNIV

Methods for generating multipotent stem cells from tonsillar biopsies

The invention provides compositions comprising multipotent progenitor cells isolated from tonsillar tissue and differentiated cells derived therefrom and methods for using the cells for the treatment of diseases or disorders.
Owner:UNIV OF MARYLAND BALTIMORE

Pig tonsil cell line susceptible to JEV

InactiveCN110468107ATroubleshooting Infected Porcine Tonsil Cell LinesGenetically modified cellsMicroorganism based processesLentivirusCulture fluid
The invention discloses a pig tonsil cell line susceptible to JEV. The cell line infects a primary pig tonsil cell line through lentivirus particles, cell screening is conducted in a serum-containingDMEM / F12 culture solution in which puromycin is added, positive cells are subjected to enlarged culture through a culture solution not containing puromycin, and the pig tonsil cell line is obtained. The lentivirus particles are obtained by taking pHY-sv4O-Puro as a lentivirus carrier and taking psPAX2 and pCMV-VSV-G as an auxiliary packaging carrier for co-transfection to enter 293T cells. The cell line has the infinite passage function, is susceptible to JEV and can be used for related research.
Owner:SHANGHAI VETERINARY RES INST CHINESE ACAD OF AGRI SCI
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