The invention discloses an end-of-line detection system of a charging pile. The end-of-line detection system is used for detecting the charging pile to be detected which is assembled on a production line, and specifically comprises a power receiving end, a power output end, a first power transmission bus, an insulation resistance testing unit, a charging gun base, a second bus, a connection confirmation detecting circuit, a control confirmation detecting circuit, a second resistor, a third resistor, an on-load test switch, and a main control device. The main control device in the end-of-line detection system can complete the insulation resistance test of the charging pile to be detected, the on-load switching on / off test, the connection confirmation signal test, the control confirmation signal test, the connection anomaly test, the display function test and the emergency stop function test, so as to ensure that the ex-factory charging piles are all qualified products, thereby preventing unqualified charging piles from damaging life and property safety of people.