The invention provides a diesel oil improver component containing biological enzyme, which comprises dispersant and biological enzyme, wherein the weight part ratio of the dispersant to biological enzyme is 1: (0.01-1); the dispersant is one of polyisobutylene succinic anhydride derivatives, Mannich base and poly alkyl polyamine; the biological enzyme is the mixture of sulpho enzyme, alcohol oxidase, aldehyde oxidase, amine oxidase, sulfhydryl oxidase, methyl mercaptan oxidase, methyl naphthoquinone oxidase, peroxidase, ferrochelatase, decarboxylase, aldolase, oxyacid lyase, demethylase, alcohol dehydratase, acid dehydratase, esters dehydratase, desulfhydrase, desulfurization methyl enzyme, cobalt chelating enzyme and magnesium chelating enzyme. The diesel oil improver component containing biological enzyme, provided by the invention, has the advantages that the high speed catalytic performance can be realized under the temperate environment through utilizing properties of the biological enzyme, and the component is combined with the conventional dispersant for use, so that cleaning efficiency of the dispersant is greatly improved, and the cleaning effect of the dispersant is improved.