The invention discloses an in vitro performance evaluating system of a continuous blood sugar monitoring sensor. The in vitro performance evaluating system comprises a reagent storage unit (1), a sampling unit (2), a sample mixing unit (3), a sample distributing unit (4), a detecting unit (5), a control unit (6), and a CGM sensor evaluating instrument (7). The in vitro performance evaluating system is capable of analyzing and evaluating the in vitro performance including sensitivity, response speed, detection range, accuracy, continuous working service life, interference resistance, temperature association coefficient, oxygen dependence degree and other parameters of the continuous blood sugar monitoring sensor as well as the performance change of the to-be-monitored sensor in a continuous working state, and is capable of effectively detecting and evaluating the in vitro detection performance of the to-be-monitored sensor.