The invention provides a growing public negative pressure room for epidemic prevention, a group and an isolation prevention and control method, a bottom support, an outer wall and a suspended ceiling are combined to form a negative pressure room body, an inner wall is arranged in the negative pressure room body, the negative pressure room body is divided into a buffer chamber, a positive chamber and an air shaft 
tube well chamber through the inner wall, and a 
fresh air machine placing cabin is arranged in the air shaft 
tube well chamber; the 
fresh air machine generates 
airflow flowing from the first air inlet end to the first air exhaust end, the first air inlet end communicates with the buffer chamber and the positive chamber, the first air exhaust end communicates with the outside of the negative pressure room body, an 
air purifier containing cabin is arranged in the air shaft 
tube well chamber, the second air inlet end communicates with the outside of the negative pressure room body, and the second air exhaust end communicates with the buffer chamber and the positive chamber. 
Fresh air blowers and 
air purifiers which act on all the buffer chambers and the positive chambers in the negative pressure room body are integrated through the air shaft 
pipe well chamber, the air pressure of the positive chambers is smaller than that of the buffer chambers, and the air pressure of the buffer chambers is smaller than that of the outside of the negative pressure room body, so that dirty 
airflow in the positive chambers cannot flow to the outside, and 
cross infection is prevented.