The invention belongs to the technical field of micro-nano manufacturing, and discloses an electrostatic focusing electro-fluid jet printing device and method. The device comprises a base, an adjusting cylinder, a nozzle and an electrostatic lens, wherein the adjusting cylinder comprises a cylinder body and a platform arranged below the cylinder body, the base is arranged in the adjusting cylinderand is fixedly connected with the adjusting cylinder through a fastening screw, moreover, a through hole is formed in the base in the vertical direction, the nozzle comprises a pinpoint and a nozzlebody for fixing the pinpoint, the nozzle body is arranged in the through hole and is fixedly connected with the base, and the electric lens comprises an upper electrode ring, an insulating ring and alower electrode ring which are sequentially arranged. The invention provides an electrostatic focusing electro-fluid jet printing method. According to the electrostatic focusing electro-fluid jet printing device and method, the electrostatic lens is integrated into an electro-fluid jet printing spray head so that higher-precision and higher-resolution printing can be achieved, moreover, printing on a insulation base plate or a free-form surface can be simultaneously achieved, the operation is simple and convenient, the printing resolution is high, and the adaptability is high.