The invention discloses a man-machine interaction method for an intelligent operation and inspection auxiliary system of a transformer substation based on a mixed reality technology. The man-machine interaction method comprises a two-dimensional code scanning module, an equipment virtual disassembly and assembly module and a real-time three-dimensional visualization module. According to the invention, the functions of equipment virtual disassembly and assembly, real-time three-dimensional visualization and remote expert assistance of the transformer substation operation and maintenance auxiliary system are realized, and the two-dimensional code is used as an entrance identifier of the module. By scanning the two-dimensional code, information of the equipment is obtained by the system, a three-dimensional model and animation of the equipment are called, and through GGV interaction, equipment virtual disassembly and assembly operations such as movement, rotation, disassembly and assembly, restoration and the like of the equipment are realized, so that operation and maintenance personnel can know the internal structure and operation method of the equipment conveniently, and the technical level, the working efficiency and the safety are improved. By scanning the two-dimensional code, the URL address is obtained and the browser is called by the system, real-time three-dimensional visualization functions such as real-time data display and over-limit alarm are achieved, and intelligent and automatic operation and maintenance are achieved.