The invention provides a pulse power supply generation circuit and an electrical dust catcher, and relates to the technical field of pulse power supplies. The circuit comprises a power supply module,a pulse generation module and a load, the pulse generation module and the power supply module are connected in series, the load is connected in parallel with two ends of the pulse generation module, the pulse generation module comprises a capacitance charging resistor, an energy storage capacitor, a transmission line charging resistor, a switch, a pulse signal generator and a high-voltage connector, the capacitance charging resistor, the transmission line charging resistor and the high-voltage connector are connected in series, one end of the energy storage capacitor is connected between the capacitance charging resistor and the transmission line charging resistor, the other end of the energy storage capacitor is connected with ground, one end of the switch is connected between the transmission line charging resistor and the high-voltage connector, the other end of the switch is connected with the ground, and the pulse signal generator is electrically connected with the switch. By thepulse power supply generation circuit, frequency-adjustable pulse strings can be generated, meanwhile, a single pulse frequency in each pulse string is also adjustable, so that the circuit is suitablefor various application occasions.