34 results about "Glycocyamine" patented technology

The invention discloses a compound rumen bypass guanidinoacetic acid additive as well as a preparation method and application thereof, and belongs to the technical field of animal feed additives. The compound rumen bypass guanidinoacetic acid additive provided by the invention is prepared from the following components in parts by mass: 40 to 60 parts of guanidinoacetic acid, 0.1 to 0.5 part of selenium yeast, 5 to 10 parts of nicotinamide, 5 to 12 parts of DL-methionine, 0.01 to 0.05 part of vitamin B12, 1 to 3 parts of compound probiotics, 3 to 5 parts of hydroxypropyl methyl cellulose, 1 to 2 parts of ethyl cellulose, 15 to 25 parts of hydrogenated palm oil and 3 to 5 parts of glyceryl monostearate. The compound probiotics comprise bacillus subtilis and saccharomyces cerevisiae. The compound additive provided by the invention can significantly improve the daily gain of ruminants and reduce the feed-gain ratio, and meanwhile, due to the improvement of the energy utilization efficiency and the enhancement of the antioxidant ability, the immunity of animals is improved, the morbidity is reduced, and the meat quality is improved.

The invention belongs to the technical field of pharmaceutical chemicals, and particularly relates to a method for preparing guanidinoacetic acid from glycine mother liquor. Based on a glycine-cyanamide method, a glycine solution containing iminodiacetic acid is used as one of reaction raw materials. When the method is used for preparing the guanidinoacetic acid, the glycine mother liquor can be directly used for preparing the guanidinoacetic acid, so that the mother liquor is directly treated, the separation step of the glycine mother liquor is omitted, and the process is simplified. Moreover, the method has an unexpected technical effect on the improvement of the yield of the guanidinoacetic acid, and the yield of the guanidinoacetic acid is improved by 6.2-6.4% compared with the prior art.

The application discloses a kind of composite guanidine group acetic acid feed nutrient additives and application, composite guanidine group acetic acid feed nutrient additives are according to dry base mass fraction, comprising the following components are made into composite granules by granulation processing: guanidine group acetic acid trace element complex: 30~70 parts;Methionine source: 20~60 parts;Carrier and binder: 5~25 parts;Wherein, the guanidine group acetic acid trace element complex is the chelation product of guanidine group acetic acid and trace element;The methionine source is selected from at least one of methionine, methionine salt, methionine hydroxyl analogue, methionine hydroxyl analogue calcium salt.This application integrates guanidine group acetic acid trace element complex, methionine and / or its hydroxyl analogue in a uniform solid particle, not only synergistic between components, and physical property uniform stable, convenient to use, can comprehensively improve the growth performance of animal, feed conversion rate and health condition.

The present invention discloses an L-arginine-glycine amidinotransferase and use thereof in the production of guanidinoacetic acid. In the present invention, through combined multi-site amino acid mutation, a technical effect of significantly improved enzyme activity of the mutant AkAGATT225Q / A258P / L278K than that of the wild-type strain is achieved, providing an application value for large-scale production of guanidinoacetic acid in industry. When the L-arginine-glycine amidinotransferase mutant constructed in the present invention is used in the production of guanidinoacetic acid, by optimizing the conversion conditions, the yield of guanidinoacetic acid is up to 21.4 g / L and the conversion rate is 90.4%, after 24 hrs of reaction in a 1 L reaction system. Compared with the production of guanidinoacetic acid with the raw enzyme, the yield is increased by 49.6%.

The present application relates to a method for preparing a granule comprising guanidinoacetic acid, the method comprising the steps of: a) providing a fermentation broth comprising guanidinoacetic acid and biomass; b) reducing the water content in the fermentation broth provided in step a) to obtain a concentrated fermentation broth; and c) subjecting the concentrated fermentation broth of step b) to wet granulation.

The invention discloses a guanidinoacetic acid N-methyltransferase mutant and application of the guanidinoacetic acid N-methyltransferase mutant in synthesis of creatine, and belongs to the technical field of bioengineering. The invention provides a method for preparing creatine by utilizing a guanidinoacetic acid N-methyltransferase mutant (Y134I / N170H) and catalyzing guanidinoacetic acid and S-adenosylmethionine in a whole-cell form. The genetically engineered bacterium for expressing the guanidinoacetic acid N-methyltransferase mutant is fermented for 24 hours under the conditions that the whole cell amount is 0.2 g, the pH value is 7.5, the reaction temperature is 37 DEG C, the concentration of guanidinoacetic acid is 80 mM, and the concentration of S-adenosylmethionine is 100 mM, the creatine yield is 7.34 g / L, and the molar yield is 69.96%. According to the method disclosed by the invention, the production cycle is shortened, the yield of creatine is increased, and the industrial process of producing creatine by an enzyme conversion method is accelerated.

The invention relates to an HPLC (High Performance Liquid Chromatography) high-efficiency quantitative method for methionine and guanidinoacetic acid in a creatine substrate synthesized by whole-cell biological catalysis. According to the method, an amino bonded silica gel chromatographic column is selected, the length of the chromatographic column is 250mm, the inner diameter of the chromatographic column is 4.6 mm, and the particle size of filler is 5mu m. A mobile phase A is 20 mmol of a dipotassium phosphate solution, the pH value is adjusted to 6.0-8.0 by using phosphoric acid, a mobile phase B is acetonitrile, and gradient elution is performed; the detection wavelength is 200 to 210 nm; the column temperature is 25-35 DEG C; the flow velocity is 0.7 to 1.0 mL / min. The detection method can be applied to accurate quantification of methionine and guanidinoacetic acid in a whole-cell biocatalytic synthesis creatine substrate, is simple in sample pretreatment, high in recovery rate, simple to operate, low in detection cost and short in detection time, has high-throughput sample detection capability, and is suitable for large-scale popularization and application. And a favorable tool is provided for exploring a safe and environment-friendly method for synthesizing creatine through industrial whole-cell biological catalysis.

For the purpose of determination for the selection of intravesical BCG instillation therapy, the present invention comprises measuring 2-ethylhydracrylic acid-2, arabinonic acid-2, carnitine, lauric acid, myristic acid, octanoylcarnitine, paraxanthine, S-methylcysteine, S-methylcysteine-S-oxide, SDMA, theobromine, indole-3-acetic acid, pentanoic acid, 1-methyl-4-imidazoleacetic acid, ADMA, alanine, argininosuccinic acid, choline, citrulline, creatinine, guanidoacetic acid, lysine, methionine sulfoxide, myristic acid, N-(1-deoxy-1-fructosyl)leucine, N-acetylneuraminic acid, N6,N6,N6-trimethyllysine, propionylcarnitine, threonine, gamma-glutamyl-glycine, gamma-glutamyl-tyrosine, and gamma-glutamyl-valine in blood, serum, or plasma.

The utility model relates to the technical field of chemical equipment, in particular to a production device of N-methylguanidinoacetic acid. According to the technical scheme, the device comprises a dissolving kettle, a mother liquor kettle, a tubular reactor, a pH adjusting kettle, a concentration kettle, a crystallization kettle and a rotary furnace, a dissolving constant-temperature heat exchanger is arranged on the outer wall of the dissolving kettle, and a dissolving liquid outlet is formed in the bottom of the dissolving kettle and connected with reaction inlets of the mother liquor kettle and the tubular reactor; a mother liquid outlet is formed in the bottom of the mother liquid kettle, a mother liquid constant-temperature heat exchanger is arranged on the outer wall, and a top mother liquid inlet is communicated with a concentrated liquid outlet in the bottom of the concentration kettle; a reaction constant-temperature heat exchanger is sleeved on the outer wall of the tubular reactor, and a reaction outlet is formed to be communicated with a top pH adjusting feeding hole of the pH adjusting kettle. The high-purity N-methylguanidinoacetic acid monohydrate is continuously produced in a continuous feeding and intermittent discharging mode, and the equipment utilization rate and the production efficiency are improved.

The present invention relates to a composition for use in the treatment and / or prevention of coccidiosis-induced conditions in poultry, wherein the composition comprises guanidinoacetic acid, guanidinoacetate or a mixture of any of these.

The present invention relates to a microorganism transformed with L-arginine:glycine amidotransferase, glyoxylate aminotransferase, and reduced malate synthase to produce guanidinoacetic acid (GAA), and a method for the fermentative production of GAA using such a microorganism. The present invention also relates to a method for the fermentative production of creatine.

The present invention relates to enzyme selection systems for glycine amidinotransferase and guanidinoacetate methyltransferase, and the use of these optimized enzyme in fermentation processes. In particular, the present invention relates to the production of creatine, using such enzymes, as well as cells optimized for creatine production.

The present application belongs to the technical field of feed additives, and particularly relates to a feed additive for improving ATP utilization rate of animal muscle cells, a preparation method and application. The feed additive comprises the following raw material components: guanidoacetic acid, methionine, betaine, vitamin E, vitamin B12, vitamin B6, folic acid, L-ascorbic acid, L-carnitine, selenium yeast and stearic acid. The present application supplements exogenous creatine synthesis raw materials for the animal body, improves the efficiency of conversion of guanidoacetic acid into creatine in the animal body, significantly increases the creatine level in the animal body, improves the ATP utilization efficiency of the animal body under the condition of lower nutrition level (low energy, low protein), reduces the conversion of nutrients such as protein into energy for consumption, and further significantly improves the growth performance and slaughter performance of the animal. The raw materials of the present application are all nutritional additives, and do not produce residues, are green and safe, meet the urgent needs of the rapid development of current antibiotic-free livestock farming, and have a wide application prospect.

The present invention belongs to the technical field of biomedicine, and specifically relates to an mRNA delivery system targeting DC cells and a preparation method therefor. The present invention innovatively grafts two molecules, mannose and guanidinoacetic acid, onto the cyclodextrin structure of polyrotaxane by means of triethylenetetramine. The grafted mannose has a DC cell-targeting effect, while the guanidino group in guanidinoacetic acid has potential cell-penetrating activity. Experimental results show that when both are simultaneously grafted onto the cyclodextrin structure of polyrotaxane, the targeting precision and delivery efficiency of the delivery system for DC cells can be synergistically enhanced. The mRNA delivery system targeting DC cells prepared by the present invention enriches the research scope of nucleic acid delivery systems and also provides strong technical support for the field of biomedicine, including tumor therapy.

The application provides a guanidoacetic acid crystal and a preparation method and application thereof, and the preparation method of the guanidoacetic acid crystal comprises the following steps: (1) mixing guanidoacetic acid crude product with water, adding an emulsifier and an acid until the guanidoacetic acid crude product is completely dissolved, then adding an alkali into the aqueous solution to react, so as to obtain a reaction solution; (2) performing stage cooling and crystallization on the reaction solution obtained in the step (1), so as to obtain the guanidoacetic acid crystal. Compared with the guanidoacetic acid crystal in the prior art, the guanidoacetic acid crystal prepared by the method has the characteristics of high purity, high fluidity and high moisture resistance, and does not need to rely on a binder and a granulation process to form a final product, and can meet the actual production needs of the feed industry.

The present invention relates to a composition for use in the treatment and / or prevention of coccidiosis-induced symptoms in poultry, comprising guanidinoacetic acid, a salt of guanidinoacetic acid, or a mixture thereof.

The invention provides an application of guanidinoacetic acid in preparation of a medicine for preventing / treating metabolism-related liver diseases, and experiments clearly prove that GAA has a remarkable treatment effect on MASH for the first time and can relieve fatty degeneration, inflammation and fibrosis of the liver; gAA has a multi-target action mechanism, can regulate lipid metabolism, inflammation and oxidative stress, and is comprehensive in curative effect; gAA is a natural metabolite, the metabolic pathway of GAA is clear, and the long-term safety of GAA is verified in the field of feed additives. Safety is high, and potential toxic and side effects are small; gAA is relocated from a nutritional supplement to a MASLD / MASH treatment drug, and a brand new treatment strategy and a drug development direction are provided for MASH.

The invention relates to the technical field of organic synthesis, and provides a zinc guanidinoacetate complex preparation method, which comprises: dissolving guanidinoacetic acid, adding a pH value adjusting agent to adjust the pH value to 3-6, adding a zinc source, and carrying out a reaction to obtain the zinc guanidinoacetate complex. Through the technical scheme, the problems of low yield and insufficient purity during preparation of the zinc guanidinoacetate complex in the prior art are solved.

This invention relates to a nonlinear optical crystal of guanidinoacetic acid chloride, its preparation method, and its uses. The crystal has the molecular formula C3N3O2H8Cl, a molecular weight of 153.57, belongs to the tetragonal crystal system, and has a space group of [missing information]. I 2 d The unit cell parameters are a =15.7150(13)Å, b =15.7150(13)Å, c =11.0742(15)Å, α =90.00° β =90.00° gamma =90.00° Z =16, V =2734.9(6)Å 3 Centimeter-sized crystals were grown using evaporation, aqueous solution cooling, or hydrothermal methods. Under 1064 nm laser irradiation, the crystal exhibited a frequency doubling response similar to that of potassium dihydrogen phosphate. The ultraviolet absorption edge of the crystal was approximately 250 nm, and the experimental birefringence was approximately 0.08 at 546 nm. This crystal preparation method is simple and low-cost, possesses significant nonlinear optical effects, a large birefringence, a wide transmission range, and is easy to grow and process. It can be used as an ultraviolet nonlinear optical crystal and a birefringent crystal.

The present invention relates to an isolated cell modified for catalyzing the conversion of guanidinoacetate acid (GAA) into creatine in the presence of glucose and methionine using an exogenous guanidinoacetate methyltransferase (GAMT) and methionine adenosyltransferase (MAT) protein, a pharmaceutical composition comprising a plurality of said isolated cells and uses thereof.

This application belongs to the field of animal nutrition and feed technology, specifically disclosing a feed for increasing creatine content in pork based on the balanced regulation of guanidinoacetic acid and amino acids, and its application. This invention provides the application of guanidinoacetic acid in preparing feed to increase creatine content in pork, and the application of the synergistic effect of guanidinoacetic acid with methionine, cystine, and lysine in preparing feed to improve pig growth performance, pork quality, and increase creatine content in pork. The feed consists of a basal diet and a guanidinoacetic acid additive, wherein the ratio of the total digestible methionine and cystine in the standard ileum of pigs to the digestible lysine in the standard ileum of pigs is 60%-70%, and the amount of guanidinoacetic acid added accounts for 0.03-0.05% of the total feed weight. This invention can effectively improve pork quality and significantly increase creatine content in pork while maintaining or improving pig growth performance.

This invention relates to the field of feed additive preparation technology, and proposes a method for preparing ethyl guanidine hydrochloride, comprising the following steps: S1, dissolving glycine ethyl ester hydrochloride in a solvent, adjusting the pH to 10-11 at 0-5°C using an alkaline adjuster to obtain a reaction solution containing free glycine ethyl ester; S2, adding a cyanamide aqueous solution to the reaction solution containing free glycine ethyl ester at 0-5°C, and reacting at 55-80°C to obtain a reaction solution; S3, after cooling the reaction solution, adjusting the pH to 6-7 using an acidic adjuster, precipitating a solid, which is then filtered and washed to obtain crude ethyl guanidine hydrochloride; S4, recrystallizing the crude ethyl guanidine hydrochloride to obtain ethyl guanidine hydrochloride. This technical solution solves the problems of low yield and low purity of ethyl guanidine hydrochloride in related technologies.

The present application relates to the field of feed, especially to a feed additive for improving meat quality of ruminants and a preparation method thereof; the present application forms functional complementation through a traditional Chinese medicine composition and guanidoacetic acid and magnesium ions; acanthopanax-scutellaria promotes body synthesis metabolism, and is synergistic with creatine precursors provided by guanidoacetic acid to optimize muscle energy reserves; schisandra chinensis and rhodiola enhance the body's stress resistance, and cooperate with magnesium ions to stabilize neuromuscular excitability and reduce excessive consumption of glycogen caused by pre-slaughter stress; polygonatum and polygonatum maintain cell membrane integrity in cooperation with vitamin E and the like; through the combined action of various components through multiple targets and multiple pathways, the synchronous improvement of muscle development and stress resistance is realized.

The invention belongs to the technical field of chemical production, and particularly relates to a method for synthesizing high-purity guanidinoacetic acid through alkali-free catalysis. Glycine and cyanamide are used as reactants, hydrophobic SBA-15 is used for constructing a hydrophobic reaction environment, and high-purity guanidinoacetic acid is obtained through nucleophilic addition-elimination reaction and refining. According to the invention, by regulating and controlling the feeding amount of glycine and utilizing the glycine to construct a proper weakly acidic reaction microenvironment, side reactions such as hydrolysis and self-polymerization of cyanamide under an alkaline condition are effectively inhibited. Meanwhile, a hydrophobically modified SBA-15 material is introduced, an ordered mesoporous structure is beneficial to adsorption and enrichment of reactants, and a hydrophobic pore channel not only can remarkably improve the concentration of glycine and cyanamide and accelerate the reaction rate, but also can timely remove water molecules generated by condensation reaction and promote forward movement of reaction balance, so that the reaction efficiency is improved. Meanwhile, the local microenvironment with less water is also beneficial to inhibiting the hydrolysis of cyanamide, so that the high-purity guanidinoacetic acid is efficiently synthesized.

The application discloses a preparation method of silicalite-1 molecular sieve and ZSM-5 molecular sieve. The molecular sieve has uniform crystal grain size and small crystal grain, which is beneficial to mass transfer and diffusion in the silicalite-1 molecular sieve and ZSM-5 molecular sieve crystals; the regular molecular sieve crystal grain is more beneficial to keeping the structure stable of the molecular sieve under the use condition and improving the service life of the molecular sieve. The method for synthesizing the silicalite-1 molecular sieve and the ZSM-5 molecular sieve by using guanidino acetic acid as an additive can prepare the silicalite-1 molecular sieve and the ZSM-5 molecular sieve crystals with the crystal grain size distribution of 140-160 nm and regular crystal shape.

To provide a method capable of determining the selection of a BCG intravesical injection therapy and total cystectomy with less burden on patients.SOLUTION: 2-ethylhydracrylic acid-2, arabinonic acid-2, carnitines, lauric acids, myristic acids, octanoylcarnitines, paraxanthine, S-methylcysteine, S-methylcysteine-S-oxide, SDMA, theobromine, indole-3-acetic acids, pentanoic acids, 1-methyl-4-imidazoleacetic acids, ADMA, alanine, argininosuccinic acids, choline, citrulline, creatinine, guanidoacetic acids, lysine, methionine sulfoxide, myristic acids, N - (1-deoxy-1-fructosyl) leucine, N-acetylneuraminic acids, N6, N6, N6 trimethyllysine, propionylcarnitines, threonine, gamma-glutamyl-glycine, gamma-glutamyl-tyrosine, the method comprises measuring gamma-glutamyl-valine.SELECTED DRAWING: Figure 1

The invention discloses rumen bypass guanidinoacetic acid microcapsules as well as a preparation method and application thereof, and belongs to the technical field of animal feed additives. The rumen bypass guanidinoacetic acid micro-capsule provided by the invention is prepared from the following components in parts by mass: 40 to 50 parts of guanidinoacetic acid, 0.5 to 2 parts of an antioxidant, 0.1 to 0.5 part of a stabilizer and 40 to 60 parts of a coating material, the coating material comprises an inner-layer coating material, a middle-layer coating material and an outer-layer coating material. By optimizing a coating material formula and a preparation process, the problems of low rumen bypass rate, poor abomasum release efficiency, insufficient stability and the like of an existing microcapsule are solved, the bioavailability of GAA in a ruminant body is improved, and the animal production performance and health condition are further improved.

The present invention provides a microorganism that has been modified to express a gene encoding a protein having arginine:glycine amidinotransferase (AGAT) activity and increase the production of glycine from L-serine by enhancing L-serine hydroxymethyltransferase activity, as well as a method for producing guanidinoacetic acid (GAA) by fermentation of such a microorganism, and a method for producing creatine.

The application discloses a method for continuously synthesizing N-methyl guanidino acetic acid and belongs to the technical field of organic synthesis. A mixed aqueous solution of cyanamide and N-methyl glycine sodium is prepared; an aqueous solution of N-methyl guanidino acetic acid is added into a reaction kettle as a reaction base material, stirring is carried out, and temperature is raised to 65-80 DEG C; the mixed aqueous solution of cyanamide and N-methyl glycine sodium is continuously added dropwise, the pH value of a reaction system is controlled to be greater than 8.0; when the volume of liquid in the reaction kettle reaches the highest liquid level set by the reaction kettle, the reaction liquid overflows into a next-stage cooling kettle; when the reaction liquid in the cooling kettle is cooled to be lower than 25 DEG C, the pH value is adjusted to be 7-8 through acidification; after being left to stand at room temperature for 2-3 h, filtration is carried out; the filter cake is recrystallized with water, dried, and then monohydrate N-methyl guanidino acetic acid is obtained. The application effectively avoids the generation of by-products such as dicyandiamide and dihydrotriazine, reduces production cost, and continuously feeds and discharges materials to replace the intermittent production mode, further reduces production cost, and is beneficial to improving market competitiveness.