45 results about "Glycocyamine" patented technology

The present invention relates to the use of creatine compounds such as, for example, creatine, creatine phosphate or analogs of creatine, such as creatine-pyruvate, creatine-ascorbate, cyclocreatine, 3 guanidinopropionic acid, guanidinoacetate, homocyclocreatine, guanidino benzoates as energy generating systems and antioxidants for preservation of skin against adverse aging effects and damage secondary to insults such as harmful sun radiations, stress and fatigue. The creatine compounds which can be used in the present method include (1) creatine, creatine phosphate and analogs of these compounds which can act as substrates or substrate analogs for creatine kinase; (2) molecules that mimic the biological activity of creatine (3) molecules that modulate the creatine kinase system.

The present invention discloses a method for synthesizing glycocyamine and salt thereof. The method for synthesizing glycocyamine salt comprises the following steps: 1) carrying out neutralization reaction on guanidine hydrochloride and an alkaline matter C for obtaining solution A; 2) facilitating chloroacetic acid to react with alkaline solution for obtaining solution B; and 3) facilitating the solution A to react with the solution B for generating glycocyamine salt. The alkaline matter C can be sodium hydroxide or sodium carbonate. Preferably the mass ratio of sodium hydroxide:guanidine hydrochloride:chloroacetic acid is 200:480:460. Preferably the mass ratio of sodium carbonate:guanidine hydrochloride:chloroacetic acid is 265:480:460. Preferably the reacting temperature of step 3) is 40 DEG C. The holding time of reaction is no less than 24 hours. The synthesis of glycocyamine refers to that the solution of step 1) directly reacts with the chloroacetic acid. The method used by the invention has the advantages of simple synthetic process, convenient operation, higher yield, less byproducts and convenient separation and purification. The product purity of the compound of the invention can be higher than 98%.

The present invention relates to the use of creatine compounds such as, for example, creatine, creatine phosphate or analogs of creatine, such as creatine-pyruvate, creatine-ascorbate, cyclocreatine, 3 guanidinopropionic acid, guanidinoacetate, homocyclocreatine, guanidino benzoates as energy generating systems and antioxidants for preservation of skin against adverse aging effects and damage secondary to insults such as harmful sun radiations, stress and fatigue. The creatine compounds which can be used in the present method include (1) creatine, creatine phosphate and analogs of these compounds which can act as substrates or substrate analogs for creatine kinase; (2) molecules that mimic the biological activity of creatine (3) molecules that modulate the creatine kinase system.

The present invention relates to the use of creatine compounds such as, for example, creatine, creatine phosphate or analogs of creatine, such as creatine-pyruvate, creatine-ascorbate, cyclocreatine, 3 guanidinopropionic acid, guanidinoacetate, homocyclocreatine, guanidino benzoates as energy generating systems and antioxidants for preservation of skin against adverse aging effects and damage secondary to insults such as harmful sun radiations, stress and fatigue. The creatine compounds which can be used in the present method include (1) creatine, creatine phosphate and analogs of these compounds which can act as substrates or substrate analogs for creatine kinase; (2) molecules that mimic the biological activity of creatine (3) molecules that modulate the creatine kinase system.

The invention discloses a chemical synthesis method of glycocyamine. The method comprises the following steps: 1. adding isopropanol, glycine and 10% of sodium hydroxide solution to a reactor and stirring the materials at room temperature until the materials are dissolved to obtain alkaline mixed solution; 2. dropwise adding cyanamide solution to the alkaline mixed solution, then carrying out stirring and reflex at certain temperature for a while, carrying out concentration under reduced pressure after reaction to vaporize a solvent to obtain the crude product of glycocyamine; and 3. throwing the crude product of guanidinobenzoic acid and water into the reactor, raising the temperature and stirring the materials and then carrying out leaching, washing and drying, thus finally obtaining glycocyamine. The chemical synthesis method provided by the invention is more suitable for industrial scale production.

Provided is a method for using low molecular weight compounds found in the body as a biomarker for diagnosing depression. Specifically, more than one compound selected from a group comprising the following are used: ADP-ribose, ATP, ADP, AMP, serotonin, tryptophan, kynurenine, SDMA (symmetrical dimethylarginine), threonine, glyceric acid, serine, N-acetylaspartic acid, glutamic acid, trigonelline, creatine, 2-methylserine, sphingosine, homovanillic acid, piperidine, sulfoxidized methionine, pipecolic acid, sphinganine, gamma-butyrobetaine, guanidinoacetic acid, isobutyric acid, creatinine, sarcosine, 3-methylbutyric acid, nicotinamide, betaine, ornithine, carnitine, ethanolamine, phosphoethanolamine, taurine, hypotaurine, aspartic acid, methionine, and tyrosine.

The invention discloses a synthesis method for glycocyamine with high purity and high yield. The synthesis method comprises the following steps: (1) adding glycine and a solvent into a reaction vessel, stirring at room temperature till glycine is dissolved completely, and adding liquid ammonia to adjust the pH value of the solution to be 10 to 11; (2) heating the mixed solution obtained in the step (1) to 55 to 60 DEG C, adding 50% hydrogen cyanamide aqueous solution into the reaction vessel slowly, stirring for 2 to 4 hours for reaction, carrying out vacuum suction filtration after the reaction is finished, and washing filter cake with filtrate to obtain a crude product of glycocyamine; (3) adding the crude product of glycocyamine obtained in the step (2) and DMF into another reaction vessel, heating to 30 to 40 DEG C, stirring for 0.5 hour, cooling to 0 DEG C, carrying out suction filtration, washing filter cake with DMF, and drying to obtain glycocyamine. The preparation method provided by the invention is simple in technology, short in production period, mild in reaction conditions, less in three wastes, high in product purity and yield, and relatively high in economical efficiency and environmental friendliness, thereby being suitable for industrialized production.

The invention provides a growth-promoting feed additive capable of improving the lean meat rate of a growing-finishing pig. The growth-promoting feed additive comprises the following components in parts by weight: 0.5-1.2 parts of nanometer-level NMDA (N-methyl-D-aspartate), 1.5-2.5 parts of glycine betaine, 7-10 parts of glycocyamine, 1-1.5 parts of Enviva EO, 40-50 parts of radix scutellariae, 20-30 parts of purslane and 15-20 parts of pine needles. The growth-promoting feed additive provided by the invention is green, healthy and pollution-free without containing antibiotics and can reduce the usage of the antibiotics, enhance the growth property of the growing-finishing pig, improve the meat quality and enhance the lean meat rate through the addition of Chinese medicinal herbs and growth-promoting regulators.

The invention belongs to the technical field of breeding, and discloses a composition of guanidinoacetic acid and a synergist thereof, a preparation method and application. The composition of the guanidinoacetic acid and the synergist thereof is prepared by uniformly mixing guanidinoacetic acid, carbohydrates, sodium chloride and methyl donor, and can be added to daily ration for feeding animals.The guanidinoacetic acid and the methyl donor are added to promote creatine synthesis, and carbohydrates and sodium chloride are supplemented to increase creatine absorption of muscle tissues, so thatmuscle protein deposition is promoted, and the problem that the production performance of livestock and poultry is reduced under an antibiotic-free condition is solved. The composition of the guanidinoacetic acid and the synergist thereof is suitable for being added into daily ration to feed animals.

The invention provides a feed additive capable of improving quality of pork and a preparation method of the feed additive. The feed additive consists of a fermented feed composition, selenium-enrichedyeast, coated cysteamine and glycocyamine. The feed additive disclosed by the invention can significantly improve meat quality and antioxidant ability, reduce feed conversion rate and improve the growing performance of fattening pigs.

The invention provides 4-percent premixed mutton sheep feed, and relates to the technical field of animal feed. The 4-percent premixed mutton sheep feed is prepared from the following ingredients in parts by weight: 31.25 parts of mutton sheep polymineral, 5 parts of mutton sheep multivitamin, 3.83 percent of 98-percent glycocyamine, 5 parts of 50-percent garlicin, 3.75 parts of 10-percent salinomycin sodium, 1.9 parts of 66-percent ethoxyquin, 86.78 parts of zeolite, 150 parts of calcium hydrophosphate, 125 parts of mountain flour, 87.5 parts of common salt, 15 parts of mulberry leaf powder, 20 parts of carrot seedlings, 15 parts of sweet potato leaf powder, 20 parts of peanut shell powder, 15 parts of locust tree powder, 2 parts of honey and 6 parts of traditional Chinese medicine additives. Compared with traditional feed, the 4-percent premixed mutton sheep feed provided by the invention has the advantages that traditional Chinese medicine ingredients are contained in the premixed feed; the traditional Chinese medicine additives and feed carriers cooperate with each other to take effects; the normal nutritional requirements of mutton sheep are ensured; in addition, the disease prevention and the disease treatment can be achieved; the immunity of the mutton sheep can be improved; in addition, the growth of the mutton sheep can also be promoted; no toxic or side effect exists; drug resistance is not generated; the environment is not polluted; the body resistance can be enhanced; the effects are fast; and the price is low.

The invention discloses an anti-stress agent for relieving transportation stress before fowl slaughtering, and a preparation method thereof. The anti-stress agent is prepared from the components in percentage by weight: 20 to 50 percent of glucose, 5 to 20 percent of guanidinoacetic acid glycine, 5 to 20 percent of glutamine, 5 to 13 percent of theanine, 5 to 10 percent of glycine betaine, 5 to 10percent of L-ascorbic acid, 1 to 5 percent of tannin, 1 to 4 percent of potassium citrate, 0 to 5 percent of sodium propionate, 0 to 5 percent of sodium diacetate, 0 to 5 percent of lactic acid, 1 to3 percent of potassium chloride, and 0 to 2 percent of sodium bicarbonate. 0.01 to 3 percent by weight of anti-stress agent is added into drinking water for fowl. The fowl drinks the anti-stress agent 48 hours before being loaded and 2 hours after being unloaded, and does not drink during the transportation process. After the fowl drinks the anti-stress agent, the moisture content of chicken canbe remarkably improved, the comprehensive stress reaction before slaughtering is reduced, the weight loss is reduced, and the economic loss is reduced.

The invention relates to an additive feed for fattening beef cattle and a use method of the additive feed. The additive feed is prepared from the following components in parts by weight: 0.05-0.2 parts of Vitamin A, 0.03-0.1 parts of vitamin D3, 0.5-1.5 parts of vitamin E, 0.5-1 part of vitamin B1, 10-15 parts of guanidinoacetic acid, 3-8 parts of glycine betaine, 0.05-0.2 parts of selenomethionine, 0.5-1 part of rumen bypass choline, 1-2 parts of ferroporphyrin, 2-5 parts of zinc sulfate, 15-20 parts of magnesium oxide, 20-30 parts of sodium sulfate, 10-18 parts of hay vinasse, 2-5 parts of potassium chloride, 1-3 parts of clostridium butyricum, 0.1-0.5 parts of an antioxidant and 20-30 parts of rice hull powder. The additive feed has the effects of promoting the rumen health of cattle and increasing the lean meat percentage of beef cattle.

The invention discloses a genetically engineered bacterium for producing guanidinoacetic acid as well as a construction method and an application of the genetically engineered bacterium. The inventionprovides the construction method of guanidinoacetic acid-producing engineering bacteria, which comprises the following steps: introducing guanidinoacetic acid synthesis-related gene L-arginine: glycine amidinyl transferase encoding gene into host bacteria to obtain guanidinoacetic acid-producing engineering bacteria; wherein the host bacteria are escherichia coli or mutant escherichia coli. According to the invention, an AGAT catalytic reaction which does not exist in the background is constructed in escherichia coli by a method of expressing foreign protein. Meanwhile, by means of plasmid overexpression of protein, replacement of promoters on chromosomes, gene knockout and the like, the flow of an ornithine circulating metabolic pathway of escherichia coli is increased, inhibition of ornithine on AGAT catalytic reaction is relieved, arginine is recycled through a whole-cell catalysis method to produce guanidinoacetic acid, and good environmental and economic prospects are achieved.

The invention discloses an anti-stress agent for relieving the transportation stress of livestock before being slaughtered and a preparation method of the anti-stress agent. The anti-stress agent is prepared from, by weight, 15-40%% of glucose, 5-25% of guanidinoacetic acid, 5-25% of glutamine, 2-15% of L-lysine, 5-10% of vitamin C, 1-12% of glycine betaine, 1-5% of potassium chloride, 1-5% of tannin, 1-5% of potassium citrate, 0-5% of calcium propionate, 0-5% of sodium butyrate, 0-5% of sodium diacetate, 0-5% of lactic acid and 1-2% of neohesperidin dihydrochalcone. The anti-stress agent is added into drinking water of the livestock according to the weight percentage of 0.01-5%, the livestock drinks the anti-stress agent 48 hours before loading and 2 hours after unloading, and does not drink the anti-stress agent during transportation. After the livestock drinks the anti-stress agent, the water content of meat can be obviously improved, the comprehensive stress reactions generated before slaughter are reduced, the weight loss is reduced, and economic losses are reduced.

The invention relates to the field of feed processing and manufacturing, and discloses a feed additive for increasing the lean meat percentage of pork. The feed additive comprises additives A and additives B. The additives A comprise Chinese herbal medicine mixed preparations, betaine, creatine monohydrate, glycocyamine, adenosine triphosphate, mixed vitamins and dopamine; the additives B comprise Chinese herbal medicine mixed preparations, amino acid, creatine monohydrate, carnitine, dihydropyridine, mixed vitamins and dopamine. The feed additive has the advantages that pigs are high in growth speed when the additives A are applied to breeding the pigs with the weights of 20-50 kilograms, the skeletal development of the pigs can be promoted by the additives A, the back-fat thickness can be reduced, and spaces can be provided for muscular synthesis; fat deposition can be accelerated when the additives B are used for breeding pigs heavier than 50 kilograms, and the deposition speeds of proteins can be increased by the additives B by means of constraints on the growth intensity of fat tissues.

The invention relates to a preparation method of coated guanidinoacetic acid. Guanidinoacetic acid and a coating material are taken as raw materials, and the coating material is sprayed on the surfaces of granular guanidinoacetic acid through a bottom spraying system of a coating machine. The preparation method comprises the following steps: (1) pretreatment; (2) pellet core preparation; (3) primary coating; and (4) secondary coating. The invention optimizes a guanidinoacetic acid coating process including selection of auxiliary materials and selection of all parameters in the preparation process. The coated guanidinoacetic acid which is good in stability and high in bioavailability can be prepared through the method. Especially for ruminants, the degradation rate of the product existing in the rumen is reduced, the slow release effect is achieved after the product passes through the rumen, and the bioavailability is high. The method is applicable to the field of feed additive preparation.

The application discloses a kit and a method for detecting related substances of genetic metabolic diseases by non-derivative tandem mass spectrometry. The kit comprises a mixed isotope internal standard substance and a quality control substance. The mixed isotope internal standard substance comprises isotope standard substances of genetic metabolic disease related substances of amino acids, carnitine, ketones, lysophosphatidylcholine, guanidinoacetic acid, creatine, adenosine and deoxyadenosine; and the quality control substance is a whole blood sample containing a quality control analyte ofthe genetic metabolic disease related substance with known concentration. The kit can detect related substances of genetic metabolic diseases of amino acids, carnitines, ketones, lysophosphatidylcholines, guanidinoacetic acid, creatine, adenosine and deoxyadenosine and thus the types of detection of related substances for genetic metabolic diseases are increased, so that screening or auxiliary screening of more metabolic diseases can be carried out in one experiment, and the use requirements of screening of the newborn genetic metabolic diseases are better met.

The invention provides fortified pig compound feed capable of reducing backfat thickness and belongs to the field of feed. The fortified pig compound feed comprises, by weight, 45-55 parts of corn, 0-20 parts of barley, 18-25 parts of soybean meal, 0-12 parts of wheat middling, 5-10 parts of rice bran, 0-8 parts of corn germ meal, 5-7 parts of premix, 0.5-1.2 parts of essential amino-acid, 0.035-0.065 part of cysteamine and 0.025-0.065 part of guanidinoacetic acid. The fortified pig compound feed has the advantages that the content of lysine, methionine, threonine and tryptophan in the feed is increased, the lysine, methionine, threonine and tryptophan are compounded with the cysteamine and guanidinoacetic acid, pig lean meat growth potential is brought into full play, pig body type is further optimized, fat meat deposition is reduced, and low feed conversion ratio is achieved.

The invention discloses an anti-stress agent for relieving fasting stress before fowl slaughtering, and a preparation method thereof. The anti-stress agent is prepared from the components in percentage by weight: 30 to 50 percent of glucose, 5 to 15 percent of guanidinoacetic acid glycine, 5 to 15 percent of glutamine, 5 to 10 percent of theanine, 5 to 10 percent of glycine betaine, 5 to 10 percent of L-ascorbic acid, 1 to 5 percent of tannin, 1 to 5 percent of potassium citrate, 1 to 5 percent of sodium propionate, 1 to 4 percent of sodium diacetate, 1 to 5 percent of lactic acid, 0.5 to 2 percent of dipotassium phosphate, and 0.5 to 2 percent of sodium bicarbonate. 0.01 to 3 percent by weight of anti-stress agent is added into drinking water for fasting fowl before slaughtering. After the fowl drinks the anti-stress agent, the moisture content of chicken can be remarkably improved, the comprehensive stress reaction before slaughtering is reduced, the weight loss is reduced, and the economic loss is reduced.

The invention discloses a preparation method of guanidinoacetic acid. The method includes: selecting ethanolamine and cyanamide as the raw materials for preparing guanidinoacetic acid, firstly reacting ethanolamine with cyanamide to obtain guanidinoethanol, then catalyzing the obtained guanidinoethanol with TEMPO to obtain a guanidinoacetic acid crude product, and then purifying the guanidinoacetic acid crude product by ethanol to obtain a guanidinoacetic acid refined product. According to the preparation method of guanidinoacetic acid, almost no side reaction occurs in the preparation processof guanidinoacetic acid, so that the generation of byproducts in the preparation process of the guanidinoacetic acid is reduced, and the difficulty of a purification process for the guanidinoacetic acid crude product is reduced. Meanwhile, the whole reaction process basically has no side reaction, so that the utilization rate of raw materials is increased, the preparation cost of the guanidinoacetic acid is reduced, and the purity of the guanidinoacetic acid prepared by adopting the method is relatively high.

The present invention pertains to a process for preparing guanidino acetic acid (GAA) from cyanamide and glycine in alkaline process solution, in which anionic impurities are removed from the process solution by electrodialysis.

The invention discloses a functional feed additive for micropterus salmoides with functions of food calling and digestion promoting and effects of remarkably reducing body fat and liver fat and improving fish body immunity. The feed additive comprises the following components in percentage by weight: 7.0% of dimethyl-beta-propiothetin (DMPT) hydrochloride, 6.0% of allicin, 10.0% of L-valine, 7.0% of betaine hydrochloride, 8.5% of guanidinoacetic acid, 16.0% of lysophosphatide powder, 5.0% of L-carnitine hydrochloride, 17.0% of an eucommia ulmoides extract and the balance carrier zeolite powder. According to the application method, in the production process, 0.3-0.9% of the additive is added into each ton of micropterus salmoides compound feed. All the components of the feed additive are natural green products, and the feed additive is stable in quality, non-toxic, harmless, free of residues, low in addition amount and easy to popularize.

A preparation method of a glycocyamine complex comprises the following steps: at first, adding glycocyamine and a trace element source substance for animals in a reaction system; carrying out reaction in a weakly alkaline environment, wherein the molar ratio of glycocyamine and a trace element is controlled to be (1.8-3.5):1; after the reaction is finished, crystallizing the reaction system, then performing solid-liquid separation to obtain a solid phase and a liquid phase, and drying to obtaining the glycocyamine complex. The glycocyamine complex prepared by the preparation method can be used as a feed additive product for application. The glycocyamine complex comprises one or more of copper glycocyamine, ferrous glycocyamine, zinc glycocyamine, manganese glycocyamine and chromium glycocyamine. When the glycocyamine complex is applied, different additive amounts can be selected according to different to-be-fed objects. The preparation method is simple in process step, low in process cost, easy to operate, has no emission or can realize clean emission, and meets the feed sanitary standard.

The present invention pertains to a process for preparing guanidino acetic acid (GAA) from cyanamide and glycine in alkaline process solution, in which anionic impurities are removed from the process solution by electrodialysis.

The invention provides a feed additive composition containing guanidinoacetic acid and a methyl donor as well as a livestock and poultry feed. The additive composition comprises the following components in parts by molar ratio: 1-10 parts of the guanidinoacetic acid, 0.5-2 parts of methionine and 0.5-5 parts of betaine. Unit of the additive composition is converted into a weight unit, and 1-10g of vitamin B6, 0.01-0.1g of vitamin B12 and 1-10g of folic acid are continuously added into each kilogram of the additive composition. Methyl required by conversion of the guanidinoacetic acid is matched with the methionine and the betaine according to a molar ratio, so that physiological conversion requirements of livestock and poultry are better met; the vitamin B6, the vitamin B12 and the folic acid are matched, so that the utilization efficiency of the methyl can be improved, cyclic transformation of homocysteine is promoted, and accumulation harm is avoided. According to the composition, methyl donor choline and a carrier are not matched, so that vitamins can be prevented from being damaged, effective content of the guanidinoacetic acid in a product is increased, and product processing and use in a feed factory are facilitated.

The invention discloses a novel efficient growth promoter. The novel efficient growth promoter comprises the following raw materials in parts by weight: 1.5-2.5 parts of 98% chromium picolinate, 55-65parts of 98% glycocyamine, 36-40 parts of 60% cysteamine, 2-5 parts of modified montmorillonite and 15-25 parts of starch coating agent. According to the efficient growth promoter disclosed by the invention, after 3 days of use, the feed intake is increased; after 7 days of use, feces is reduced, and buttocks start to become plump; after 15 days of use, rumps are developed, bodily forms are beautiful and strong, the flesh color is ruddy, backs are broadened, and dorsal furrows are deep; and after about 20 days of use, all regions of pigs, flocks and herds already reach plump bodily forms.

The invention relates to a method for refining glycocyamine. The method comprises the steps as follows: A. certain quantity of crude glycocyamine is dispersed in water to prepare turbid glycocyamine solution; B. certain quantity of sodium hyposulfite is dissolved in the water to prepare sodium hyposulfite aqueous solution; C. the sodium hyposulfite aqueous solution obtained in step B is dropwise added into the turbid glycocyamine solution obtained in step A and simultaneously the turbid glycocyamine solution is stirred and heated to react for a period of time; D. mixed solution obtained in step C is cooled and filtered to collect solid substance; and E. the solid substance obtained in step D is dried to obtain a refined glycocyamine product. The method for refining the glycocyamine can prepare the refined glycocyamine product with the purity more than 99 percent.

The present invention discloses a method for synthesizing glycocyamine and salt thereof. The method for synthesizing glycocyamine salt comprises the following steps: 1) carrying out neutralization reaction on guanidine hydrochloride and an alkaline matter C for obtaining solution A; 2) facilitating chloroacetic acid to react with alkaline solution for obtaining solution B; and 3) facilitating thesolution A to react with the solution B for generating glycocyamine salt. The alkaline matter C can be sodium hydroxide or sodium carbonate. Preferably the mass ratio of sodium hydroxide:guanidine hydrochloride:chloroacetic acid is 200:480:460. Preferably the mass ratio of sodium carbonate:guanidine hydrochloride:chloroacetic acid is 265:480:460. Preferably the reacting temperature of step 3) is 40 DEG C. The holding time of reaction is no less than 24 hours. The synthesis of glycocyamine refers to that the solution of step 1) directly reacts with the chloroacetic acid. The method used by theinvention has the advantages of simple synthetic process, convenient operation, higher yield, less byproducts and convenient separation and purification. The product purity of the compound of the invention can be higher than 98%.