61 results about "Glycocyamine" patented technology

The invention discloses a compound rumen bypass guanidinoacetic acid additive as well as a preparation method and application thereof, and belongs to the technical field of animal feed additives. The compound rumen bypass guanidinoacetic acid additive provided by the invention is prepared from the following components in parts by mass: 40 to 60 parts of guanidinoacetic acid, 0.1 to 0.5 part of selenium yeast, 5 to 10 parts of nicotinamide, 5 to 12 parts of DL-methionine, 0.01 to 0.05 part of vitamin B12, 1 to 3 parts of compound probiotics, 3 to 5 parts of hydroxypropyl methyl cellulose, 1 to 2 parts of ethyl cellulose, 15 to 25 parts of hydrogenated palm oil and 3 to 5 parts of glyceryl monostearate. The compound probiotics comprise bacillus subtilis and saccharomyces cerevisiae. The compound additive provided by the invention can significantly improve the daily gain of ruminants and reduce the feed-gain ratio, and meanwhile, due to the improvement of the energy utilization efficiency and the enhancement of the antioxidant ability, the immunity of animals is improved, the morbidity is reduced, and the meat quality is improved.

The invention provides an additive containing amino acid complexed potassium and magnesium as well as a preparation method and application of the additive, and relates to the technical field of feed additives. The additive containing amino acid complexed potassium magnesium is prepared from the following raw materials in parts by weight: 45-55 parts of amino acid complexed potassium magnesium, 15-25 parts of sorbitol, 5-15 parts of sodium citrate, 2-4 parts of guanidinoacetic acid, 2-4 parts of dihydropyridine and 10-20 parts of glucose, wherein the amino acid complexed potassium magnesium is prepared from amino acid, potassium hydroxide and magnesium chloride. The prepared additive containing amino acid complexed potassium and magnesium can improve the anti-stress capability of chickens, can reduce the feed conversion ratio and the death rate when being used in chicken feed, reduces the damage to the chickens caused by stress reaction, and also can reduce the expression of inflammatory factors and improve the expression of anti-inflammatory cytokines, thereby improving the immunity of the organism.

The invention discloses a preparation method and application of a finishing agent based on guanidinoacetic acid, and relates to the technical field of textiles. According to the finishing agent prepared by the corresponding method, the used guanidinoacetic acid is an amino acid analogue and can be used as a food additive, so that the finishing agent is harmless to a human body in a use process, and the finishing agent is simple in preparation method, short in synthesis period, simple in post-treatment, high in yield and easy for industrial production; according to the finishing agent disclosed by the invention, guanidinoacetic acid is applied to the field of fabric finishing for the first time, and after the finishing agent based on guanidinoacetic acid is applied to the fabric, the finished fabric is endowed with three properties, namely flame retardance, hydrophobicity and ultraviolet resistance, and the mechanical properties of the finished fabric are enhanced.

The invention belongs to the technical field of pharmaceutical chemicals, and particularly relates to a method for preparing guanidinoacetic acid from glycine mother liquor. Based on a glycine-cyanamide method, a glycine solution containing iminodiacetic acid is used as one of reaction raw materials. When the method is used for preparing the guanidinoacetic acid, the glycine mother liquor can be directly used for preparing the guanidinoacetic acid, so that the mother liquor is directly treated, the separation step of the glycine mother liquor is omitted, and the process is simplified. Moreover, the method has an unexpected technical effect on the improvement of the yield of the guanidinoacetic acid, and the yield of the guanidinoacetic acid is improved by 6.2-6.4% compared with the prior art.

The present invention belongs to the field of biomedicine technology, and specifically relates to an mRNA delivery system targeting DC cells and a preparation method thereof. The present invention innovatively grafts two molecules, mannose and guanidine acetic acid, onto the cyclodextrin structure of a polyrotaxane through triethylenetetramine. The grafted mannose has a DC cell targeting effect, and the guanidine group in guanidine acetic acid has a potential cell penetration effect. Experimental results show that when the two are grafted onto the cyclodextrin structure of a polyrotaxane at the same time, the targeting accuracy and delivery efficiency of the delivery system for DC cells can be synergistically improved. The mRNA delivery system targeting DC cells prepared by the present invention not only enriches the research scope of nucleic acid delivery systems, but also provides strong technical support for biomedical fields such as tumor treatment.

The application discloses a kind of composite guanidine group acetic acid feed nutrient additives and application, composite guanidine group acetic acid feed nutrient additives are according to dry base mass fraction, comprising the following components are made into composite granules by granulation processing: guanidine group acetic acid trace element complex: 30~70 parts;Methionine source: 20~60 parts;Carrier and binder: 5~25 parts;Wherein, the guanidine group acetic acid trace element complex is the chelation product of guanidine group acetic acid and trace element;The methionine source is selected from at least one of methionine, methionine salt, methionine hydroxyl analogue, methionine hydroxyl analogue calcium salt.This application integrates guanidine group acetic acid trace element complex, methionine and / or its hydroxyl analogue in a uniform solid particle, not only synergistic between components, and physical property uniform stable, convenient to use, can comprehensively improve the growth performance of animal, feed conversion rate and health condition.

The invention relates to a transforming microorganism capable of producing guanidinoacetic acid (GAA), which is improved by using carbamate kinase (2.7. 2.2) and L-arginine: glycine amidino transferase (2.1. 4.1), and a method for producing GAA by using the microorganism through fermentation. The microorganism can be further improved by deleting a central repressor protein argR, expressing glyoxylate aminotransferase (2.6. 1.-) or expressing NADH dependent amino acid dehydrogenase (1.4. 1.-). The invention also relates to a method for the fermentative production of creatine by further expression of guanidinoacetic acid N-methyltransferase (2.1. 1.2).

The present invention discloses an L-arginine-glycine amidinotransferase and use thereof in the production of guanidinoacetic acid. In the present invention, through combined multi-site amino acid mutation, a technical effect of significantly improved enzyme activity of the mutant AkAGATT225Q / A258P / L278K than that of the wild-type strain is achieved, providing an application value for large-scale production of guanidinoacetic acid in industry. When the L-arginine-glycine amidinotransferase mutant constructed in the present invention is used in the production of guanidinoacetic acid, by optimizing the conversion conditions, the yield of guanidinoacetic acid is up to 21.4 g / L and the conversion rate is 90.4%, after 24 hrs of reaction in a 1 L reaction system. Compared with the production of guanidinoacetic acid with the raw enzyme, the yield is increased by 49.6%.

The present application relates to a method for preparing a granule comprising guanidinoacetic acid, the method comprising the steps of: a) providing a fermentation broth comprising guanidinoacetic acid and biomass; b) reducing the water content in the fermentation broth provided in step a) to obtain a concentrated fermentation broth; and c) subjecting the concentrated fermentation broth of step b) to wet granulation.

The invention relates to detection and quantification of guanidinoacetic acid, creatine and creatinine by mass spectrometry. Methods of detecting or determining the amounts of guanidinoacetic acid (GAA), creatine, and creatinine by mass spectrometry are provided.

The invention discloses a guanidinoacetic acid N-methyltransferase mutant and application of the guanidinoacetic acid N-methyltransferase mutant in synthesis of creatine, and belongs to the technical field of bioengineering. The invention provides a method for preparing creatine by utilizing a guanidinoacetic acid N-methyltransferase mutant (Y134I / N170H) and catalyzing guanidinoacetic acid and S-adenosylmethionine in a whole-cell form. The genetically engineered bacterium for expressing the guanidinoacetic acid N-methyltransferase mutant is fermented for 24 hours under the conditions that the whole cell amount is 0.2 g, the pH value is 7.5, the reaction temperature is 37 DEG C, the concentration of guanidinoacetic acid is 80 mM, and the concentration of S-adenosylmethionine is 100 mM, the creatine yield is 7.34 g / L, and the molar yield is 69.96%. According to the method disclosed by the invention, the production cycle is shortened, the yield of creatine is increased, and the industrial process of producing creatine by an enzyme conversion method is accelerated.

The invention relates to an HPLC (High Performance Liquid Chromatography) high-efficiency quantitative method for methionine and guanidinoacetic acid in a creatine substrate synthesized by whole-cell biological catalysis. According to the method, an amino bonded silica gel chromatographic column is selected, the length of the chromatographic column is 250mm, the inner diameter of the chromatographic column is 4.6 mm, and the particle size of filler is 5mu m. A mobile phase A is 20 mmol of a dipotassium phosphate solution, the pH value is adjusted to 6.0-8.0 by using phosphoric acid, a mobile phase B is acetonitrile, and gradient elution is performed; the detection wavelength is 200 to 210 nm; the column temperature is 25-35 DEG C; the flow velocity is 0.7 to 1.0 mL / min. The detection method can be applied to accurate quantification of methionine and guanidinoacetic acid in a whole-cell biocatalytic synthesis creatine substrate, is simple in sample pretreatment, high in recovery rate, simple to operate, low in detection cost and short in detection time, has high-throughput sample detection capability, and is suitable for large-scale popularization and application. And a favorable tool is provided for exploring a safe and environment-friendly method for synthesizing creatine through industrial whole-cell biological catalysis.

The invention belongs to the technical field of feed additives, and particularly relates to a feed additive for reducing carcass fat of fattening bulls as well as a preparation method and application of the feed additive. The feed additive for reducing the carcass fat of the fattening bulls is prepared from the following raw materials in parts by weight: 10 to 35 parts of coated polysaccharide cinnamyl aldehyde, 2 to 5 parts of recombinant alphas2-casein, 0.5 to 2 parts of glaucocalyxin B, 1 to 15 parts of creatine phosphate, 5 to 25 parts of guanidinoacetic acid, 5 to 25 parts of methionine, 5 to 25 parts of vitamin B1 and 15 to 60 parts of wheat bran. Wherein the mass fraction of the polysaccharide cinnamyl aldehyde in the coated polysaccharide cinnamyl aldehyde is 45-50%; the amino acid sequence of the recombinant alpha s2-casein is as shown in SEQ ID NO. 1. When the feed additive is added into the feed, the cattle body weight can be obviously increased, the cattle carcass fat deposition and the feed-gain ratio are reduced, the fattening feed conversion efficiency is improved, and the feed additive has important significance in improving the economic value of the fattening cattle.

For the purpose of determination for the selection of intravesical BCG instillation therapy, the present invention comprises measuring 2-ethylhydracrylic acid-2, arabinonic acid-2, carnitine, lauric acid, myristic acid, octanoylcarnitine, paraxanthine, S-methylcysteine, S-methylcysteine-S-oxide, SDMA, theobromine, indole-3-acetic acid, pentanoic acid, 1-methyl-4-imidazoleacetic acid, ADMA, alanine, argininosuccinic acid, choline, citrulline, creatinine, guanidoacetic acid, lysine, methionine sulfoxide, myristic acid, N-(1-deoxy-1-fructosyl)leucine, N-acetylneuraminic acid, N6,N6,N6-trimethyllysine, propionylcarnitine, threonine, gamma-glutamyl-glycine, gamma-glutamyl-tyrosine, and gamma-glutamyl-valine in blood, serum, or plasma.

The utility model relates to the technical field of chemical equipment, in particular to a production device of N-methylguanidinoacetic acid. According to the technical scheme, the device comprises a dissolving kettle, a mother liquor kettle, a tubular reactor, a pH adjusting kettle, a concentration kettle, a crystallization kettle and a rotary furnace, a dissolving constant-temperature heat exchanger is arranged on the outer wall of the dissolving kettle, and a dissolving liquid outlet is formed in the bottom of the dissolving kettle and connected with reaction inlets of the mother liquor kettle and the tubular reactor; a mother liquid outlet is formed in the bottom of the mother liquid kettle, a mother liquid constant-temperature heat exchanger is arranged on the outer wall, and a top mother liquid inlet is communicated with a concentrated liquid outlet in the bottom of the concentration kettle; a reaction constant-temperature heat exchanger is sleeved on the outer wall of the tubular reactor, and a reaction outlet is formed to be communicated with a top pH adjusting feeding hole of the pH adjusting kettle. The high-purity N-methylguanidinoacetic acid monohydrate is continuously produced in a continuous feeding and intermittent discharging mode, and the equipment utilization rate and the production efficiency are improved.

The present invention relates to a composition for use in the treatment and / or prevention of coccidiosis-induced conditions in poultry, wherein the composition comprises guanidinoacetic acid, guanidinoacetate or a mixture of any of these.

The present invention relates to a microorganism transformed with L-arginine:glycine amidotransferase, glyoxylate aminotransferase, and reduced malate synthase to produce guanidinoacetic acid (GAA), and a method for the fermentative production of GAA using such a microorganism. The present invention also relates to a method for the fermentative production of creatine.

The present invention relates to enzyme selection systems for glycine amidinotransferase and guanidinoacetate methyltransferase, and the use of these optimized enzyme in fermentation processes. In particular, the present invention relates to the production of creatine, using such enzymes, as well as cells optimized for creatine production.

The present application belongs to the technical field of feed additives, and particularly relates to a feed additive for improving ATP utilization rate of animal muscle cells, a preparation method and application. The feed additive comprises the following raw material components: guanidoacetic acid, methionine, betaine, vitamin E, vitamin B12, vitamin B6, folic acid, L-ascorbic acid, L-carnitine, selenium yeast and stearic acid. The present application supplements exogenous creatine synthesis raw materials for the animal body, improves the efficiency of conversion of guanidoacetic acid into creatine in the animal body, significantly increases the creatine level in the animal body, improves the ATP utilization efficiency of the animal body under the condition of lower nutrition level (low energy, low protein), reduces the conversion of nutrients such as protein into energy for consumption, and further significantly improves the growth performance and slaughter performance of the animal. The raw materials of the present application are all nutritional additives, and do not produce residues, are green and safe, meet the urgent needs of the rapid development of current antibiotic-free livestock farming, and have a wide application prospect.

A method for preparing guanidino acetic acid involves reacting cyanamide and glycine in an aqueous reaction mixture in the presence of a base. The ammonia content in the reaction mixture is controlled to be below 20 g / L, and the dicyandiatrade content in the reaction mixture is kept below 5 wt.-%.

The present invention belongs to the technical field of biomedicine, and specifically relates to an mRNA delivery system targeting DC cells and a preparation method therefor. The present invention innovatively grafts two molecules, mannose and guanidinoacetic acid, onto the cyclodextrin structure of polyrotaxane by means of triethylenetetramine. The grafted mannose has a DC cell-targeting effect, while the guanidino group in guanidinoacetic acid has potential cell-penetrating activity. Experimental results show that when both are simultaneously grafted onto the cyclodextrin structure of polyrotaxane, the targeting precision and delivery efficiency of the delivery system for DC cells can be synergistically enhanced. The mRNA delivery system targeting DC cells prepared by the present invention enriches the research scope of nucleic acid delivery systems and also provides strong technical support for the field of biomedicine, including tumor therapy.

A microorganism is transformed to be capable of producing guanidinoacetic acid (GAA). A method can be used for the fermentative production of GAA using such microorganism. A corresponding method can also be used for the fermentative production of creatine.

The application provides a guanidoacetic acid crystal and a preparation method and application thereof, and the preparation method of the guanidoacetic acid crystal comprises the following steps: (1) mixing guanidoacetic acid crude product with water, adding an emulsifier and an acid until the guanidoacetic acid crude product is completely dissolved, then adding an alkali into the aqueous solution to react, so as to obtain a reaction solution; (2) performing stage cooling and crystallization on the reaction solution obtained in the step (1), so as to obtain the guanidoacetic acid crystal. Compared with the guanidoacetic acid crystal in the prior art, the guanidoacetic acid crystal prepared by the method has the characteristics of high purity, high fluidity and high moisture resistance, and does not need to rely on a binder and a granulation process to form a final product, and can meet the actual production needs of the feed industry.

The present invention relates to a microorganism capable of producing guanidinoacetic acid (GAA) after transformation, said microorganism comprising at least one gene encoding a protein having the function of NADH-dependent dehydrogenase, and a method for fermentative production of GAA using such a microorganism. The invention also relates to a method for producing creatine by fermentation.

The invention belongs to the technical field of guanidinoacetic acid, and particularly relates to a granulation method of guanidinoacetic acid and guanidinoacetic acid particles. A glycine solution with a mass concentration of 20% to 40% and a binder are mixed to prepare a mixed solution A, wherein the mass concentration of the binder in the mixed solution A is 1% to 5%; sodium hydroxide is added to the mixed solution A, and the mixture is heated to dissolve to obtain a clear solution B; a cyanamide solution is dripped into the clear solution B to obtain a reaction solution C after the dripping is completed, wherein the molar ratio of cyanamide to glycine in the cyanamide solution is (0.8 to 1):1; the reaction solution C is cooled to 40 to 50°C and then kept warm for 1 to 2 hours, and then cooled to 10 to 12°C again after the heat preservation, and centrifuged to obtain guanidinoacetic acid particles, wherein the cooling rate is 5°C / h to 10°C / h, and the process of preparing the guanidinoacetic acid particles is short and simple, and does not require the aid of granulation equipment, and has the beneficial effect of reducing costs and increasing efficiency.

The present invention relates to a method for the fermentative production of guanidinoacetic acid (GAA) using a microorganism transformed to be capable of producing GAA, which is improved by using carbamate kinase (2.7.2.2) and L-arginine:glycine amidinotransferase (2.1.4.1), and such a microorganism that can be further improved by deleting the central repressor protein argR and expressing glyoxylate aminotransferase (2.6.1.-) or NADH-dependent amino acid dehydrogenase (1.4.1-). The present invention also relates to a method for the fermentative production of creatine by further expressing guanidinoacetic acid N-methyltransferase (2.1.1.2).

A microorganism is capable of producing guanidinoacetic acid (GAA) that has been improved by using a carbamate kinase. The microorganism with an improved capacity to provide L-arginine as starting material of the GAA biosynthesis by efficient recycling of ornithine improves the production process of GAA. A method for the fermentative production of GAA and a method for the fermentative production of creatine include the incorporation of the microorganism.

The present invention relates to a composition for use in the treatment and / or prevention of coccidiosis-induced symptoms in poultry, comprising guanidinoacetic acid, a salt of guanidinoacetic acid, or a mixture thereof.

The invention provides an application of guanidinoacetic acid in preparation of a medicine for preventing / treating metabolism-related liver diseases, and experiments clearly prove that GAA has a remarkable treatment effect on MASH for the first time and can relieve fatty degeneration, inflammation and fibrosis of the liver; gAA has a multi-target action mechanism, can regulate lipid metabolism, inflammation and oxidative stress, and is comprehensive in curative effect; gAA is a natural metabolite, the metabolic pathway of GAA is clear, and the long-term safety of GAA is verified in the field of feed additives. Safety is high, and potential toxic and side effects are small; gAA is relocated from a nutritional supplement to a MASLD / MASH treatment drug, and a brand new treatment strategy and a drug development direction are provided for MASH.