The invention provides a method, a
system, equipment and a medium for improving SPP
microscopic imaging resolution, and the method comprises the steps: carrying out the annular shaping of light emitted by a
light source through a conical lens combination, obtaining a collimated annular
light spot, and focusing the collimated annular
light spot to a rear focal plane of an objective lens; the collimated annular
light spot is obliquely incident to a
metal-substrate interface of an excitation
chip in an annular wide-field
plane wave mode, the incident angle is adjusted to be an SPP excitation angle, Bessel SPP is excited on the surface of a
gold film of the excitation
chip, and the Bessel SPP is spread along the surface of the
gold film and is scattered with a nano substance; sPP scattering is converted into an optical
signal, the optical
signal and reflected light are collected by an objective lens together, transmitted to a far field through a
leaky mode radiation effect and imaged in a
CCD camera, so that the problems that the SPRM imaging spatial resolution is low due to anisotropic transmission during one-way SPP excitation, and the SPRM real-time
rapid imaging is affected due to switching of the SPP excitation direction through a mechanical device are solved.