The invention discloses a two-stage wake-up circuit applicable to an electronic toll collection (ETC) system, and relates to integrated circuit technique. The two-stage wake-up circuit applicable to the ETC system comprises a primary wake-up circuit and a secondary wake-up circuit. The primary wake-up circuit is low in power consumption and can work all the time under a standby state. The secondary wake-up circuit is moderate in sensitivity, good in stability, strong in process-voltage-temperature resisting ability, capable of accurately judging that whether an input signal is an ETC wake-up signal or not, but high in power consumption, the secondary wake-up circuit is in a dormant state under the standby state, and waking-up and dormancy of the secondary wake-up circuit are controlled by the primary wake-up circuit. According to the two-stage wake-up circuit, the primary wake-up circuit and the secondary wake-up circuit are combined to be used, so that the whole ETC two-stage wake-up circuit not only has the advantage of the primary wake-up circuit of being low in power consumption, but also has the advantages of the secondary wake-up circuit of being moderate in the sensitivity, good in the stability, low in mistake wake-up rate and the like. Meanwhile, a low-power-consumption wake signal amplifying circuit with no external polarization required is adopted in the primary wake-up circuit, and therefore power consumption of the primary wake-up circuit is lowered.