The invention provides an optical tweezers type optical fiber Raman microprobe and a manufacturing method. The probe has two optical channels which are coaxial, wherein an annular optical fiber core provides a Raman exciting light channel and a channel in the coaxial center is used for receiving Raman probe light; and by performing fine taper angle grinding on the fiber ends of coaxial dual waveguide channel optical fibers, a rotary symmetrical plane (or chambered surface) structure is formed. The structure can converge Raman exciting light transmitted by the annular core in a micron order, and on the one hand, the converged exciting light has an ability of capturing micron-order and nanoscale particles and on the other hand, the converged exciting light interacts with the particles, so that a back scattering Raman optical signal generated by the exciting light converged by the Raman scattering light can be collected and transmitted to a Raman spectrometer through an intermediate large-core fire cores. The microprobe provided by the invention can capture micro living matters of cell living bodies to effectively excite the Raman spectrum of the matters in cells and to obtain the Raman spectrum so as to achieve Raman measurement of micro liquids, single cells in living bodies and inner substances thereof.