The invention discloses a polyaniline type normal-temperature blackening agent as well as a preparation method and application thereof, and relates to the technical field of metal surface treatment. The polyaniline type normal-temperature blackening agent is prepared from the following components in parts by weight: 0.1 to 1.5 parts of copper sulfate pentahydrate, 0.1 to 2.5 parts of sodium tungstate dihydrate, 0.5 to 12 parts of polyaniline reaction liquid, 0.05 to 1.2 parts of nickel sulfate, 0.1 to 1.3 parts of sodium thiosulfate, 0.05 to 1.2 parts of disodium 1-hydroxyethylidene-1, 1-diphosphonate, 0.01 to 1.0 part of fatty alcohol-polyoxyethylene ether and a proper amount of deionized water. In the components, copper sulfate pentahydrate, sodium tungstate dihydrate and polyaniline reaction liquid are used as main film-forming substances, nickel sulfate is used as a secondary film-forming substance, sodium thiosulfate is used as a film-forming aid, disodium 1-hydroxyethylidene-1, 1-diphosphonate is used as a chelating agent, and fatty alcohol-polyoxyethylene ether is used as a dispersion stabilizer. The finally formed blackening film can cut off the direct connection between metal and oxygen, meanwhile, the continuous protection effect can be achieved, and under the combined action of all the components, the corrosion resistance is obviously improved.