The invention discloses a long-acting moisturizing composition as well as a preparation method and application thereof. The long-acting moisturizing composition is prepared from polyol, water-soluble ceramide, bio-saccharide gum-1, tremella polysaccharide, oat beta-glucan, hydroxyethyl urea, saccharide isomeride, an ecological nutrient solution and water. According to the long-acting moisturizing composition disclosed by the invention, the polyhydric alcohol, the water-soluble ceramide, the bio-saccharide gum-1, the tremella polysaccharide, the oat beta-glucan, the hydroxyethyl urea, the saccharide isomeride and the ecological nutrient solution are used as raw materials to be compounded to prepare the long-acting moisturizing composition, and a very good synergistic effect is generated among the raw materials, so that the water content in skin can be increased after the long-term use of the long-acting moisturizing composition; therefore, the long-acting moisturizing effect is achieved.