The utility model provides a portable science popularization experiment teaching aid box, and relates to the portable science popularization experiment teaching aid box technology field, the portable science popularization experiment teaching aid box comprises a box, a baffle plate is arranged in the box, the surface of the baffle plate is provided with an adjusting device, the side surface of the box is provided with a binding device, the inner wall of the box is fixedly connected with a display panel, and the inner wall of the box is fixedly connected with the display panel. A box cover is rotatably connected to the upper top of the box, the adjusting device comprises a rectangular groove formed in the upper top of a baffle, a sliding plate is slidably connected to the interior of the rectangular groove, adjusting holes of different sizes are formed in the surface of the sliding plate, a through groove is formed in the surface of the baffle, and a groove is formed in the box; the inner part of the groove is fixedly connected with a fixed rod, the surface of the fixed rod is in sliding connection with a sliding block, the upper top part of the sliding block is fixedly connected with a baffle plate, and the problem that the hole of the baffle plate in the pinhole imaging cannot be adjusted is solved.