The invention relates to the field of PCB plug-in assembly, in particular to a PCB plug-in feeding system for guiding installation through utilizing laser. The PCB plug-in feeding system comprises a PCB, and further comprises a rack, a positioning and installing mechanism, a laser guiding mechanism, a feeding mechanism, a material taking mechanism and an electric control system, wherein an inclined operation platform is arranged in the middle of the rack, the positioning and installing mechanism comprises a PCB carrier platform, the laser guiding mechanism is arranged at the top end of the rack, the laser guide mechanism is provided with a laser emitter capable of freely moving in the X-axis direction and the Y-axis direction, the feeding mechanism comprises a plurality of material supply boxes capable of freely moving in the X-axis direction and the Y-axis direction, and the positioning and installing mechanism, the laser guiding mechanism, the feeding mechanism and the material taking mechanism are all electrically connected with the electric control system. According to the PCB plug-in feeding system, a laser designator, a linkage mechanical conveying device and an intelligent material supplementing system are utilized, materials needed by laser indication are automatically supplied, the probability of wrong insertion is completely eradicated, the production efficiency is improved, the pre-warning material supplementing function is achieved, and lean production is achieved.