The invention discloses a modified amino organic fluorosilicone oil emulsion color-deepening finishing agent. The color-deepening finishing agent is characterized by being prepared from, by mass, 50%-95% of octamethylcyclotetrasiloxane, 1%-30% of 1,3,5-trimethyltris-1,5-(3,3-trifluoropropyl)-cyclotrisiloxane, 1%-20% of N-(beta-aminoethyl)-gamma-aminopropyl methyl dimethoxy silicane, 0.01%-0.1% of an end capping agent, 0.01%-0.1% of a high-alkalinity catalyst, 0.1%-2% of an emulsifying agent and the balance deionized water. The modified amino organic fluorosilicone oil emulsion color-deepening finishing agent has the following structural formula (please see the formula in the description), wherein X ranges from 10 to 100, Y ranges from 80 to 1,000, Z ranges from 7 to 70, and X, Y and Z are all integers. A preparation method has the advantages that the raw materials are easy to obtain, and operation is convenient. The color-deepening finishing agent prepared through the method is used for finishing a real silk fabric, the finished real silk fabric has the excellent color fixing property and washing resistance, the reflected light amount on the fiber surface is decreased, and the color-deepening effect is obvious.