The invention discloses a production process of jacquard fabric with a petit point effect and belongs to the technical field of jacquard weaving. The production process comprises specification design,fabric weave design and looming weaving, wherein a warp combination comprises a larger number of surface warp and a smaller number of lining warp, a weft combination is formed by weft A, weft B and weft C, the looming warp density and the looming weft density are 112.8 pieces/cm and 114 pieces/cm separately, the proportion of the weft A, the weft B and the weft C is 1:1:1, a single loom beam is adopted for weaving, ground weave is double-layer weave, surface-layer weave is through hole weave, lining-layer weave is plain weave, a surface layer is formed by interweaving the weft A and the weftB both in the opening state with the surface warp, a lining layer is formed by interweaving the weft C at the other opening with the lining-layer warp, and the weft C is partially connected with the surface-layer warp. The jacquard fabric having high warp and high weft density and prepared with the process has remarkable petit-point texture and a good weaving yarn hole effect.