The invention relates to a method for measuring
vitamin A, D, and E in milk
powder, which comprises the following steps: mixing the milk
powder sample evenly, weighing an appropriate amount, adding water, absolute
ethanol,
ascorbic acid, BHT and KOH
aqueous solution, heating and saponifying, and
saponification is completed After cooling to constant volume; using a high-performance liquid chromatograph equipped with a double-valve three-pump
system to measure the content of
vitamin A, D, and E in the solution; by comparing the
retention time of the sample and the standard substance in the one-dimensional liquid
chromatography, confirm that the
vitamin A, D, and E in the sample Whether it contains vitamin A, E; by comparing the
retention time of the sample and the standard substance in the two-dimensional liquid
chromatography, confirm whether the sample contains vitamin D; finally, according to the obtained amount of vitamin A, D, E, calculate the vitamin in the sample after conversion A, D, E content; the invention simplifies the operation steps, greatly shortens the pretreatment time, saves manpower and
reagent costs, realizes automatic online purification detection, improves sensitivity, and ensures the accuracy and reproducibility of test results.