The invention relates to a method for measuring vitamin A, D, and E in milk powder, which comprises the following steps: mixing the milk powder sample evenly, weighing an appropriate amount, adding water, absolute ethanol, ascorbic acid, BHT and KOH aqueous solution, heating and saponifying, and saponification is completed After cooling to constant volume; using a high-performance liquid chromatograph equipped with a double-valve three-pump system to measure the content of vitamin A, D, and E in the solution; by comparing the retention time of the sample and the standard substance in the one-dimensional liquid chromatography, confirm that the vitamin A, D, and E in the sample Whether it contains vitamin A, E; by comparing the retention time of the sample and the standard substance in the two-dimensional liquid chromatography, confirm whether the sample contains vitamin D; finally, according to the obtained amount of vitamin A, D, E, calculate the vitamin in the sample after conversion A, D, E content; the invention simplifies the operation steps, greatly shortens the pretreatment time, saves manpower and reagent costs, realizes automatic online purification detection, improves sensitivity, and ensures the accuracy and reproducibility of test results.