The invention discloses a preparation method of a multilayer BST (Barium Strontium Titanate) film based on gradient pre-crystallization heat treatment, belonging to the technical field of functional materials. According to the preparation method, in a process of preparing a multilayer BST film by layers, the BST prepared by layers are subjected to gradient pre-crystallization heat treatment. The BST film based on the gradient pre-crystallization heat treatment has the characteristics of growth along a crystal surface (110), constant and low growth speed, average crystal grain of 20-30 nm, clear crystal boundary, small grain gap, smoothness and compactness, no cracks, no shrinkage cavity, small dielectric temperature coefficient, stable frequency characteristic, and remarkably improved comprehensive dielectric performance. A dielectric constant is 322-398, the tuning rate is 34.5-46.3 percent, the dielectric loss is 0.55-0.97 percent, the leakage current density is 4.2*10<-9>-9.0*10<-8>A / cm<2>, the dielectric temperature coefficient is 1.3*10<-3>-3.4*10<-3> / K, the high-quality factor is 42.9-76.1, and the microwave practicability of the BST film can be met.