The invention relates to a novel negative pressure gas-liquid separator, which is mainly composed of: a gas outlet flange, a gas-liquid mixture inlet flange, a top cover, a hand wheel, an upper box body, a tapered ring, an upper rubber valve core, a lining board, an upper valve base, a lower box body, a lower valve base board, a lower valve base, a lower valve core, a lower conical ring, an upperconical ring, a baffle, an upper valve base board, and a liquid outlet, etc. The upper box body is in bolt connection with the lower box body. The gas outlet flange is connected to an external exhaustfan or a vacuum pump. An overflow device composed of the baffle and valve bases are arranged in the lower box body. By means of the manual valve and the overflow device, liquid seal is achieved at aliquid outlet essentially, so that technical problems that air enters the separator from the liquid outlet and installation position of the separator is limited since there is no throttling or forcedliquid discharge apparatus at the liquid outlet in the prior art can be solved, thereby increasing gas-liquid separation efficiency.