32 results about "Chemical fingerprinting" patented technology

The invention relates to an identification method of precious rosewood based on chemical fingerprint. The method provided by the invention is based on the chemical composition of the wood and can identify the precious rosewood at molecular level by virtue of chemometrics. The method includes ultrasonic extraction, nitrogen-blow concentration, etc. Sample chromatogram of the precious rosewood is collected by GC-FID, then PCA projection is carried out on the obtained chromatogram after being pretreated, thus obtaining identification result. The method realizes identification on unknown rosewood sample, and the result is accurate and reliable.

The invention relates to a quality assurance and identification method for high-quality strawberries, belonging to the technical field of biology. The quality assurance and identification method comprises the following steps: determining phenols and flavor substances of different varieties of strawberries by virtue of HPLC-MS and GC-MS, and establishing a chemical fingerprint chromatography database; determining the total antioxidant activity of the various strawberries by virtue of an ORAC method; analyzing blastogenesis diversity of the strawberries by utilizing RFLP, RAPD and SSR methods and combining key enzyme genes in synthetic routes of anthocyanin and polyphenol; establishing a spectrum-effect relationship between chemical fingerprint chromatography and biological activity information of the different varieties of strawberries, and analyzing the relation between hereditary basis and functional quality character of the strawberries. The high-quality variety of the strawberries is selected by combining chemical fingerprint chromatography, the effectiveness evaluation, genetic diversity analysis and the spectrum-effect relationship and is subjected to authenticity identification, so that a scientific, reliable, uniform and standard strawberry quality evaluation system is established, and technical support and scientific basis are provided for the quality control of the strawberries.

An identification method of the rhizome gastrodiae includes the below processes: (1) setting the standard chemical fingerprint atlas of rhizome gastrodiae: a. putting the rhizome gastrodiae standard sample into the sorbitic extractor; b. adding the ethanol and putting in the water bath for hot reflux, treating it by the ultrasonic cell knapper and weight, then to loss weight by the corresponding solvent; repeating it again; after filtration, concentration and drying, it uses the 10% acetonitrile to constant volume; c. using the HPLC, Eclipse, XD13-C8 chromatographic column, the 10% acetonitrile solution is as the mobile phase, the light of 221+-1nm wavelength is as the detection light source to determine the chemical fingerprint atlas of rhizome gastrodiae of rhizome gastrodiae standard sample; which has seven evidence peaks, the peak with 1.920min holding time is the rhizome gastrodiae element peak, the rhizome gastrodiae element peak of the other rhizome gastrodiae has the big warp of 1.9% with the standard sample. (2) Determining the chemical fingerprint of the detected sample and the dummy sample according to the progress (1) and comparing to identify.

The invention discloses a construction method for electrochemical fingerprint of tea. The method constructs the electrochemical fingerprint of tea by using an electrochemical work station in virtue of chemical oscillation technology. The construction method is characterized in that tea crushed to a certain particle size is used as a raw material; on the premise that the bulk volume of a reaction is 100 mL, certain amounts of H2SO4 and MnSO4 and 1 mL of CH3COCH3 are successively added and isothermal stirring is carried out at a certain temperature for 10 min; then a certain amount of KBrO3 is added; the time when the first drop of KBrO3 is added is taken as starting time, and the electrochemical work station is used for recording the electrochemical fingerprint of the tea. Through comparison of similarity of electrochemical fingerprint of tea, tea from different producing areas and of different varieties can be discriminated.

The present invention relates to a pawpaw electrochemical fingerprint identification method, provides a method for identifying the authenticity of pawpaw through an electrochemical fingerprint, and belongs to the field of traditional Chinese medicine quality control. The method comprises: A, establishing a pawpaw chemical component fingerprint, wherein pawpaw with the same production place and the same brand is adopted as a standard substance, the electrochemical fingerprint of all component is established through an oscillation reaction, and results are shown in figure 1; and B, detecting a sample, wherein a pawpaw sample to be detected is detected under the same conditions as the standard substance so as to obtain the fingerprint of the detected sample, and the fingerprints of the standard substance and the detected sample are analyzed through a direct observation method or Origin software analysis so as to be adopted as the qualitative basis. According to the present invention, all components of the pawpaw are made into the fingerprint, the method has advantages of good reproducibility, no requirement of pretreatment, simple and fast operation, economy and the like, can provide reliable information for the identification of the quality of all types of pawpaw and the identification of the low-price pawpaw and the high-price pawpaw, and is suitable for promotion in the technical field of pawpaw safety detection.

Solution of sample to be tested in arenobufagin class is obtained through following steps: using high performance liquid chromatography, UV detector, chromatographic work station, the method places arenobufagin sample 25mg to be tested into conical flask with plug; adding methyl alcohol 20ml, weighing up fixed weight; heating up reflow for 1h, cooling and weighing up fixed weight again, using methyl alcohol to supplement weight reduced; shaking to uniformity and passing 0.2 micro filter membrane. Chemical finger print of specific bufotenine class and bufogenin class in arenobufagin class is obtained from following steps: under condition of mobile phase, buffer solution of methyl alcohol, gradient elution is carried out at analytical column; sample size as 20 micro liter, retention time 90min. chemical attribution is carried out for all or most of characteristic peak. The method prepares fingerprint of arenobufagin correctly so as to provide reliable reference for identification.

The invention discloses a construction method and applications of a tussilago farfara l HPLC fingerprint, and relates to the technical field of construction of fingerprints. The construction method comprises: (1) extracting tussilago farfara l by using methanol, and filtering with a filtration membrane to obtain a test solution; (2) detecting with a high pressure liquid chromatograph to obtain a chemical fingerprint; and (3) analyzing the chemical fingerprint, wherein the results determine that the tussilago farfara l liquid phase fingerprint contains 14 characteristic peaks. According to the present invention, the construction method has advantages of simple and convenient sample pretreatment and good reproducibility, can accurately reflect the whole quality characteristics of the herb tussilago farfara l, and is used for the quality control of the herb tussilago farfara l.

The invention relates to a detection method for obtaining material composition and total antioxidant activity of lonicera confusa. The detection method comprises the following steps: preparing a test solution; preparing a DPPH solution; preparing a series of concentration gradient VC solution as a standard substance, carrying out HPLC-DPPH analysis by taking DPPH as a to-be-scavenged object, and establishing a regression equation of relationship between VC concentration and free radical scavenging rate; and injecting the test solution into the HPLC-DPPH analysis system to obtaining the chemical ingredient information of a sample and the total free radical scavenging rate of the sample, and substituting into the regression equation, so that the total antioxidant activity of the sample is obtained. The detection method provided by the invention has the advantages that an HPLC-DPPH online combination method is adopted, and chemical fingerprint chromatography information and the total antioxidant activity of the lonicera confusa can be obtained at the same time, so that the advantage of carrying out quality control and 'spectrum-effect' evaluation on the lonicera confusa is realized.

The present invention discloses a construction method and applications of a Shanxi mature vinegar liquid-phase fingerprint, and relates to the technical field of fingerprint construction. The construction method comprises: (1) taking 1 mL of Shanxi mature vinegar, and filtering by using a 0.22 [mu]m micro-pore filtration membrane for spare; and (2) sucking the filtered Shanxi mature vinegar, and determining through a high-pressure liquid chromatograph to obtain the chemical fingerprint. According to the present invention, the sample pre-treatment is simple and easy, the chemical components and the quality characteristics of the Shanxi mature vinegar can be completely reflected, the Shanxi mature vinegar liquid-phase fingerprint can be used for the quality control of the Shanxi mature vinegar and can further provide technical support of the protection of the Shanxi mature vinegar having the origin production region and the geographical indication.

The invention relates to component proportions and a screening method of chemical components of sodium aescinate and relates to the field of pharmacy. The screening method comprises the following steps: obtaining sodium aescinate samples with different component proportions; performing detection to obtain a chemical fingerprint chromatogram of the samples, the dosage of the samples to test bodiesas well as corresponding irritation test data and pharmacodynamic test data; performing grey correlation analysis to obtain a first analytic result of an irritation sequence and a pharmacodynamic sequence of all components of the sodium aescinate; constructing a neural network by taking the dosage as an input layer and taking the irritation test data and the pharmacodynamic test data which are subjected to normalized weighting treatment as an output layer; predicting all components of the sodium aescinate by using the trained neural network, and then, performing comparison with the first analytic result; and performing screening and verification. The component proportions and the screening method provided by the invention have the advantages that the screening is fast and effective, the screening time is effectively shortened, the screening cost is lowered, sodium aescinate chemical components with low irritation and good pharmacodynamic effects are obtained, and medicinal effects andclinical compliance are improved.

A method for testing acanthopanax bark features that the simple ultrasonic extracting in methanol is combined with the capillary electrophoresis type electrochemical analysis method, and the quality of acanthopanax bark is determined by comparing the contents of active components and the fingerprint.

The invention belongs to the technical field of spectrum processing, and discloses a processing method and system of a quantized fingerprint spectrum of a spectrum, a medium and application of the quantized fingerprint spectrum of the spectrum. Quantum fingerprint transformation is carried out on continuous spectrum signal points or non-separation chromatographic signal points by adopting an interval erasing method, a feature degeneration method, a fixed-point merging method and an intelligent quantizing method; and linear or rod-like quantum fingerprints for spectral or non-separation chromatography are obtained, the information is merged and stored on the quantum fingerprint peak points obtained by merging, and overall qualitative and overall quantitative calculation of the quantum fingerprints can be performed according to the obtained quantum fingerprint peak information in combination with fingerprint software, so that the quantum fingerprints can be used for an evaluated target entity. According to the invention, a continuous spectrum and a non-separation chromatography can be converted into a linear or rod-shaped quantized fingerprint spectrum mode; and meanwhile, a chromatogram, a chromatogram with incomplete separation and other continuous physical signals, electrochemical fingerprints and continuous time signal curves which can represent the content or activity of substances can be converted into linear or rod-shaped quantized fingerprints.

The invention relates to a method for rapidly identifying pericarpium citri reticulatae and citrus chachiensis hortorum through an electrochemical fingerprint spectrum, and belongs to the field of identification of traditional Chinese medicinal materials. Factors influencing identification are screened through analytic hierarchy process, and three factors including temperature, sulfuric acid and sodium bromate are selected according to the importance degree for an experiment; the optimal experiment condition is selected through a single-factor experiment, and finally the pericarpium citri reticulatae and the citrus chachiensis hortorum are identified under the optimal condition. Under the condition, not only are time and cost saved, but also the pericarpium citri reticulatae and the citrus chachiensis hortorum are obviously different. The technology provides a technical basis for identification and quality evaluation of pericarpium citri reticulatae, citrus chachiensis hortorum and other similar traditional Chinese medicinal materials in the future.

The invention relates to a serum pharmacochemistry preparation method of a Chinese medicinal weight-reducing prescription. Obesity can cause a series of diseases, such as hypertension, diabetes mellitus, heart diseases, cerebrovascular diseases, cancers, cholecystitis, infections and the like. At present, clinical medicaments for treating the obesity are all brain appetite inhibitor and have higher side effects after long-term use, thus, the clinical medicaments are only used in a short term in a limitation manner in most countries. The traditional Chinese medicine has originality for treating the obesity in China, and most weight-reducing prescriptions are low in toxic or side effects and are safe and effective in long-term use. According to the invention, a traditional Chinese medicine serum pharmacochemistry method is intended to be used, two or three effective Chinese medicinal weight-reducing prescriptions are finely selected by using a chemical fingerprint technology and a targeted gene high-throughput sieving technology, multi-target-point weight-reducing genes are used to determine bioactivity, and active compounds are traced and separated, so that the foundation for developing a new weight-reducing medicine is laid.

The invention provides a method for detecting bird's nest by electrochemical fingerprint spectrum, which adopts B-Z oscillation reaction detection, and the specific operation steps are as follows: (1) preparation of bird's nest hydrolyzate; (2) B-Z electrochemical oscillation fingerprint spectrum; (3) Analysis of electrochemical fingerprints. The method for detecting bird's nest by electrochemical fingerprint of the present invention adopts BrO 3 ‑ ‑Ce 2+ ‑H + ‑The identification of bird's nest by malonic acid oscillation system can obtain characteristic information without complicated pretreatment. It is an accurate, simple and low-cost identification method with good application prospects.

The invention discloses a method for converting a chemical fingerprint chromatography into a two-dimensional code in traditional Chinese medicine quality tracing. Data files exported after detection of an instrument are converted into the same Excel formatted files ended with xls in a unified mode; the content of the data files is read, denoising processing is conducted, all kinds of unnecessary data are removed, and rounding is conducted on an absorbancy column; all feature point sets are found; one digit after the decimal point is reserved for a time column, and data deduplication is conducted with the time column as the benchmark; the final processed feature point data set is obtained; the final data set is converted into the two-dimensional code. The method solves the problem that the information amount of traditional Chinese medicine chemical fingerprint chromatography information is large, the traditional Chinese fingerprint chromatography can be successfully converted into the two-dimensional code through the algorithm, and the blank home and abroad is filled up.

The invention relates to an electrochemical fingerprint chromatogram identification method for edible oil and provides a method for identifying the authenticity of the edible oil through electrochemical fingerprint chromatograms, belonging to the field of edible oil inspection method. The method comprises the following steps of: a) establishing an edible oil chemical composition fingerprint chromatogram: taking edible oil of the same origin and the same brand as a standard product and establishing the electrochemical fingerprint chromatogram of all chemical compositions of the standard product, wherein the result is shown as the accompanying drawing 1; and b) inspecting a sample: taking an edible oil sample to be inspected, inspecting the edible oil sample under the same conditions under which the standard product is inspected to obtain the chromatogram of the inspected sample, and using a direct observation method or origin software to analyze the fingerprint chromatograms of the standard product and the edible oil sample to obtain a result which is used as a qualitative basis. By using the fingerprint chromatogram of all compositions used by the edible oil, the method has the advantages of good repeatability, no pretreatment, simplicity and convenience in operation, rapidness, economy and the like. Reliable information can be provided for the quality identification of various edible oil products and the identification of low-price edible oil which pretends to be high-price edible oil. The method can be widely popularized in the technical field of edible oil safety inspection.

The present invention discloses a construction method and applications of a Shanxi mature vinegar liquid-phase fingerprint, and relates to the technical field of fingerprint construction. The construction method comprises: (1) taking 1 mL of Shanxi mature vinegar, and filtering by using a 0.22 [mu]m micro-pore filtration membrane for spare; and (2) sucking the filtered Shanxi mature vinegar, and determining through a high-pressure liquid chromatograph to obtain the chemical fingerprint. According to the present invention, the sample pre-treatment is simple and easy, the chemical components and the quality characteristics of the Shanxi mature vinegar can be completely reflected, the Shanxi mature vinegar liquid-phase fingerprint can be used for the quality control of the Shanxi mature vinegar and can further provide technical support of the protection of the Shanxi mature vinegar having the origin production region and the geographical indication.

In this invention we have characterized plant extracts derived from common plants used in Indian traditional medicine using a) chemical fingerprinting by HPLC and b) etiology based in-vitro cell assays in order to reduce the extracts to minimum constituent to use for therapeutic purpose against degenerative conditions like cardiovascular diseases, Diabetes mellitus, Hypertension, All types of Cancers, Obesity & weight problems, Arthritis & pain, Alzheimer's disease, Multiple sclerosis & Autoimmune diseases, Asthma & Allergies, Osteoporosis & bone health, Parkinson's disease, Stress, Disorders & diseases of the eye. The current status of the extracts is that all of them have been chemically fingerprinted and have gone through assays to screen anti-diabetic activity. Thus currently we are applying for the specific leads that have shown very specific anti-diabetic activity. However we are also working in parallel, to test these extracts in other assays, for generating information of there applicability in the prevention or treatment of degenerative conditions as mentioned above. As and when we will have data to support disease specific application of individual extracts we will amend our claims accordingly. The present invention relates to a composition for curing or treating degenerative disorders, a process for the preparation of the same, and usage of the same and, more particularly, to the composition having as a major component a single extract or multiple extracts containing therapeutically relevant bioactive, obtained from a certain kind of plant / plants and being capable of lowering or reducing a level of glucose in the blood of a patient suffering from diabetes mellitus by alleviating or ameliorating various symptoms caused by or associated with the diabetes mellitus as well as preventing the glucose level in the blood of the diabetic patient with high certainty from arising again. The invention also relates to the in-vitro screening of plant extracts for anti-diabetic activities in multiple cell based and biochemical systems. Studies have shown eleven potential leads, which are very valuable as they could lead to bioactives having therapeutic activity in the treatment of like cardiovascular diseases, Diabetes mellitus, Hypertension, All types of Cancers, Obesity & weight problems, Arthritis & pain, Alzheimer's disease, Multiple sclerosis & Autoimmune diseases, Asthma & Allergies, Osteoporosis & bone health, Parkinson's disease, Stress, Disorders & diseases of the eye.

The invention relates to the field of traditional Chinese medicine quality tracing, in particular to a method and a device of conversion of a two-dimensional code. The method comprises following steps of: respectively obtaining DNA sequences and chemical fingerprint chromatography data of a traditional Chinese medicine; screening out chemical fingerprint chromatography data satisfying a preset rule form the chemical fingerprint chromatography data, carrying out shaping processing on the screened-out chemical fingerprint chromatography data, and according to the size of the chemical fingerprint chromatography data subjected to the shaping processing, combining the chemical fingerprint chromatography data subjected to the shaping processing into an array storing required smallest byte size; processing the DNA sequences according to a first preset compression algorithm, and combining the processed DNA sequences and the combined chemical fingerprint chromatography data according to a set format; and converting the combined DNA sequences and the chemical fingerprint chromatography data into a two-dimensional code. Therefore, a problem of large amount of information of traditional Chinese medicines is solved; compression rate is improved; and the DNA sequences and the chemical fingerprint chromatography data are successfully converted into the two-dimensional code.

The invention relates to an identification method of precious rosewood based on chemical fingerprint. The method provided by the invention is based on the chemical composition of the wood and can identify the precious rosewood at molecular level by virtue of chemometrics. The method includes ultrasonic extraction, nitrogen-blow concentration, etc. Sample chromatogram of the precious rosewood is collected by GC-FID, then PCA projection is carried out on the obtained chromatogram after being pretreated, thus obtaining identification result. The method realizes identification on unknown rosewoodsample, and the result is accurate and reliable.

The invention relates to a quality assurance and identification method for high-quality strawberries, belonging to the technical field of biology. The quality assurance and identification method comprises the following steps: determining phenols and flavor substances of different varieties of strawberries by virtue of HPLC-MS and GC-MS, and establishing a chemical fingerprint chromatography database; determining the total antioxidant activity of the various strawberries by virtue of an ORAC method; analyzing blastogenesis diversity of the strawberries by utilizing RFLP, RAPD and SSR methods and combining key enzyme genes in synthetic routes of anthocyanin and polyphenol; establishing a spectrum-effect relationship between chemical fingerprint chromatography and biological activity information of the different varieties of strawberries, and analyzing the relation between hereditary basis and functional quality character of the strawberries. The high-quality variety of the strawberries is selected by combining chemical fingerprint chromatography, the effectiveness evaluation, genetic diversity analysis and the spectrum-effect relationship and is subjected to authenticity identification, so that a scientific, reliable, uniform and standard strawberry quality evaluation system is established, and technical support and scientific basis are provided for the quality control of the strawberries.

The invention discloses a method for identifying genuineness and fake of lycium ruthenicum based on a nonlinear chemical fingerprint spectrum technology. The method comprises the following steps of respectively adding sulfate, manganese sulfate, acetone and sodium bromate solutions into lycium ruthenicum standard products from each production place, placing into a nonlinear chemical fingerprint spectrum intelligent analyzer, obtaining nonlinear chemical fingerprint spectrums of lycium ruthenicum from each production place, performing equal-length standard treatment on the nonlinear chemical fingerprint spectrums, and extracting feature parameter information; under the same condition of standard products, obtaining the nonlinear chemical fingerprint spectrum and feature parameter information of to-be-tested samples; according to the maximum relative standard deviation, visual shape difference and integral similarity of the fingerprint spectrums of the standard products and the to-be-tested samples, dividing the to-be-tested samples into genuine products, doubted products and fake products. The method has the advantages that the complicated pretreatment steps of separating, extracting and the like are not needed, the cost of the equipment is low, the whole content of all active ingredients can be represented in a cluster way, and the detection cost is low.

The invention relates to the field of traditional Chinese medicine quality tracing, in particular to a method and a device of conversion of a two-dimensional code. The method comprises following steps of: respectively obtaining DNA sequences and chemical fingerprint chromatography data of a traditional Chinese medicine; screening out chemical fingerprint chromatography data satisfying a preset rule form the chemical fingerprint chromatography data, carrying out shaping processing on the screened-out chemical fingerprint chromatography data, and according to the size of the chemical fingerprint chromatography data subjected to the shaping processing, combining the chemical fingerprint chromatography data subjected to the shaping processing into an array storing required smallest byte size; processing the DNA sequences according to a first preset compression algorithm, and combining the processed DNA sequences and the combined chemical fingerprint chromatography data according to a set format; and converting the combined DNA sequences and the chemical fingerprint chromatography data into a two-dimensional code. Therefore, a problem of large amount of information of traditional Chinese medicines is solved; compression rate is improved; and the DNA sequences and the chemical fingerprint chromatography data are successfully converted into the two-dimensional code.

The present invention is quality analysis and identification method of herbal medicine material glabrous sarcandra herb and its three kinds of Chinese medicine preparation, compound glabrous sarcandrabuccal tablet, Qingrexiaoyanning capsule and Xuekang oral liquid. The method is one capillary electrophoresis-electrochemical analysis process, which includes the main steps of comparing the capillary electrophoresis-electrochemical analysis obtained fingerprints for the quantitative detection of the components, and identifying the Chinese medicine based on peak number, peak height and peak height ratio. The present invention has the advantages of high separation efficiency, low detection limit, low cost, high reproducibility, etc.

The invention belongs to the technical field of edible oil detection, and particularly relates to an edible oil electrochemical fingerprint identification method which comprises the following steps: taking edible oil as a standard sample; putting the edible oil, an H2SO4 solution, a malonic acid solution and a sodium bromide solution into a reaction tank, and adding a nonionic surfactant into the mixed solution; starting a computer, a millivoltmeter and a magnetic stirrer which are connected with a data acquisition device, and starting timing; starting a super thermostat, adjusting the temperature in the reaction tank, and rapidly adding ceric ammonium sulfate and a sodium bromate solution through an injector; taking a to-be-detected edible oil sample to obtain a spectrum of the detected sample, directly observing the fingerprints of the edible oil sample and the detected sample, and taking Origin software analysis as a qualitative basis; and determining a sample atlas of the to-be-detected edible oil. According to the invention, the phenomenon of interference on the detection result due to the existence of reducing substances in the detection process is reduced, the detection accuracy is improved, and meanwhile, the detection speed is also improved.

The invention belongs to the technical field of traditional Chinese medicine fingerprint analysis, and discloses a method for detecting and analyzing active sites of anti-pulmonary fibrosis of Periplaneta americana L.. The method comprises the following steps of: B, constructing a ML-HB fingerprint, constructing a spectrum-activity relationship regression equation by combining chromatographic peakarea values of the ML-HB fingerprint using partial least squares method, and selecting a common peak positively correlated with pharmacological activity in the chromatographic peak of the ML-HB of the active sites of anti-pulmonary fibrosis of the Periplaneta americana L.. According to the method for detecting and analyzing the active sites of anti-pulmonary fibrosis of the Periplaneta americanaL., the HPLC-ELSD fingerprint of the ML-HB of the active sites of anti-pulmonary fibrosis of the Periplaneta americana L. and the spectrum-activity relationship inhibiting the growth of HEL cells thereof are established, and a relationship between information in a chemical fingerprint and a pharmacological activity can be better reflected compared to a simple chemical component fingerprint, so that a new idea is provided for a quality control study of the ML-HB.