The invention discloses an automatic assembling technology and device of a microwave filter tuning screw. The device comprises a machine frame module, a gasket feeding module, a nut feeding module, a tuning screw feeding module, a machine vision module, a platform module and an assembling mechanical arm module. A nut, a gasket and a screw are used for finishing the feeding action at the same time, the efficiency is extremely high, and the nut, the gasket and the screw are positioned at the same time through detachable product jig; the two-step strategy is adopted in the feeding method of the screw, and by means of a material pipe, the whole automatic assembling device is not directly provided with vibrating discs comprising the tuning screw, the structure is simple, the stability of the whole device is high, and the stability and the reliability of the whole device are prevented from being affected by vibration of the vibrating discs; the defects that the efficiency of an existing manual work assembling mode is low, the error rate is high and the homogeneity of products is poor are overcome, and a foundation is laid for the follow-up automatic adjusting resonant frequency procedures of a microwave filter.