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34 results about "Resource poor" patented technology

Methods and algorithms for cell enumeration in a low-cost cytometer

The enumeration of cells in fluids by flow cytometry is widely used across many disciplines such as assessment of leukocyte subsets in different bodily fluids or of bacterial contamination in environmental samples, food products and bodily fluids. For many applications the cost, size and complexity of the instruments prevents wider use, for example, CD4 analysis in HIV monitoring in resource-poor countries. The novel device, methods and algorithms disclosed herein largely overcome these limitations. Briefly, all cells in a biological sample are fluorescently labeled, but only the target cells are also magnetically labeled. In addition, non-magnetically labeled cells are imaged for viability in a modified slide configuration. The labeled sample, in a chamber or cuvet, is placed between two wedge-shaped magnets to selectively move the magnetically labeled cells to the observation surface of the cuvet. An LED illuminates the cells and a CCD camera captures the images of the fluorescent light emitted by the target cells. Image analysis performed with a novel algorithm provides a count of the cells on the surface that can be related to the target cell concentration of the original sample. The compact cytometer system provides a rugged, affordable and easy-to-use technique, which can be used in remote locations.
Owner:UNIVERSITY OF TWENTE

A simple and affordable method for immunophenotyping using a microfluidic chip sample preparation with image cytometry

The enumeration of cells in fluids by flow cytometry is widely used across many disciplines such as assessment of leukocyte subsets in different bodily fluids or of bacterial contamination in environmental samples, food products and bodily fluids. For many applications the cost, size and complexity of the instruments prevents wider use, for example, CD4 analysis in HIV monitoring in resource-poor countries. The novel device, methods and system disclosed herein largely overcome these limitations. The system includes a simple system for CD4 and CD8 counting in point-of-care HIV staging within resource poor countries. Unlike previous approaches, no sample preparation is required with the sample added directly to a chip containing dried reagents by capillary flow. A large area image cytometer consisting of an LED module is used to excite the fluorochromes PerCP and APC labeled targets and a monochrome CCD camera with a combination of two macro lenses captures images of 40 mm2 of blood (approximately 1 microliter). CD4 and CD8-T-lymphocyte counts correlate well with those obtained by flow cytometry. The cytometer system described in the present invention provides an affordable and easy-to-use technique for use in remote locations.
Owner:UNIVERSITY OF TWENTE

Methods and algorithms for cell enumeration in a low-cost cytometer

The enumeration of cells in fluids by flow cytometry is widely used across many disciplines such as assessment of leukocyte subsets in different bodily fluids or of bacterial contamination in environmental samples, food products and bodily fluids. For many applications the cost, size and complexity of the instruments prevents wider use, for example, CD4 analysis in HIV monitoring in resource-poor countries. The novel device, methods and algorithms disclosed herein largely overcome these limitations. Briefly, all cells in a biological sample are fluorescently labeled, but only the target cells are also magnetically labeled. In addition, non-magnetically labeled cells are imaged for viability in a modified slide configuration. The labeled sample, in a chamber or cuvet, is placed between two wedge-shaped magnets to selectively move the magnetically labeled cells to the observation surface of the cuvet. An LED illuminates the cells and a CCD camera captures the images of the fluorescent light emitted by the target cells. Image analysis performed with a novel algorithm provides a count of the cells on the surface that can be related to the target cell concentration of the original sample. The compact cytometer system provides a rugged, affordable and easy-to-use technique, which can be used in remote locations.
Owner:UNIVERSITY OF TWENTE

Methods and Algorithms For Cell Enumeration in a Low-Cost Cytometer

The enumeration of cells in fluids by flow cytometry is widely used across many disciplines such as assessment of leukocyte subsets in different bodily fluids or of bacterial contamination in environmental samples, food products and bodily fluids. For many applications the cost, size and complexity of the instruments prevents wider use, for example, CD4 analysis in HIV monitoring in resource-poor countries. The novel device, methods and algorithms disclosed herein largely overcome these limitations. Briefly, all cells in a biological sample are fluorescently labeled, but only the target cells are also magnetically labeled. In addition, non-magnetically labeled cells are imaged for viability in a modified slide configuration. The labeled sample, in a chamber or cuvet, is placed between two wedge-shaped magnets to selectively move the magnetically labeled cells to the observation surface of the cuvet. An LED illuminates the cells and a CCD camera captures the images of the fluorescent light emitted by the target cells. Image analysis performed with a novel algorithm provides a count of the cells on the surface that can be related to the target cell concentration of the original sample. The compact cytometer system provides a rugged, affordable and easy-to-use technique, which can be used in remote locations.
Owner:VERIDEX LCC

Method for concentrating and recovering noble metals from printed circuit boards of discarded mobile phones and catalysts of discarded cars using discarded nonferrous slag

The present invention relates to a method for concentrating and recovering noble metals from printed circuit boards of discarded mobile phones and catalysts of discarded cars using discarded nonferrous slag, which is industrial waste discharged from a process for refining nonferrous metals such as copper, lead, zinc and the like, and more specifically, to a method for concentrating and recovering gold, silver, platinum, palladium, rhodium and the like contained in printed circuit boards of discarded mobile phones and catalysts of discarded cars by melting discarded nonferrous slag, printed circuit boards of discarded mobile phones and catalysts of discarded cars at a high temperature through a single process to reduce and separate iron oxide contained in the discarded nonferrous slag and simultaneously melting and separating copper, iron, tin, and nickel contained in the printed circuit boards of discarded mobile phones to use the generated iron, copper, tin, and nickel alloy as a collector metal for noble metals. The method for concentrating and recovering noble metals from printed circuit boards of discarded mobile phones and catalysts of discarded cars using discarded nonferrous slag of the present invention comprises the steps of: mixing and melting discarded nonferrous slag and a solvent, which is a slag composition controller; inserting printed circuit boards of discarded mobile phones and catalysts of discarded cars to the obtained molten metal to melt the same; and maintaining the same for a predetermined amount of time to separate the same into a noble metal-collected alloy phase and a slag phase containing no noble metals. In addition, the present invention relates to a method for recovering valuable metals such as iron, copper, tin, nickel and the like in addition to noble metals such as gold, silver, platinum, palladium, and rhodium from printed circuit boards of discarded mobile phones and catalysts of discarded cars, and recycling the generated slag without environmental problems. According to the present invention, the amount of the generated alloy phase is increased without using a collector metal for noble metals such as copper, iron, lead, and nickel and carbon as a reducing agent which increase processing costs by using discarded nonferrous slag, which is industrial waste discharged from a process for refining nonferrous metals such as copper, lead, zinc, and the like, as a solvent, which is a slag composition controller, and a noble metal collector simultaneously, and using a plastic component contained in printed circuit boards of discarded mobile phones as a reducing agent, and thus an alloy phase and a slag phase can be readily separated, thereby simultaneously reducing processing time and minimizing the amount of a solvent such as alumina (Al2O3), quicklime (CaO), magnesia (MgO), iron oxide (FeO) and silica (SiO2). Accordingly, noble metals such as gold, silver, platinum, palladium, rhodium and the like can be concentrated and recovered through a single process by simultaneously treating different industrial waste such as printed circuit boards of discarded mobile phones and catalysts of discarded cars, and waste material can be recycled to be used as a material for the high-technology industry, and thus it is possible to maximize the coefficient of utilization of noble metal resources in resource-poor Korea, which depends on imports for all of noble metal resources.
Owner:KOREA INST OF GEOSCI & MINERAL RESOURCES

Methods and algorithms for cell enumeration in a low-cost cytometer

The enumeration of cells in fluids by flow cytometry is widely used across many disciplines such as assessment of leukocyte subsets in different bodily fluids or of bacterial contamination in environmental samples, food products and bodily fluids. For many applications the cost, size and complexity of the instruments prevents wider use, for example, CD4 analysis in HIV monitoring in resource-poor countries. The novel device, methods and algorithms disclosed herein largely overcome these limitations. Briefly, all cells in a biological sample are fluorescently labeled, but only the target cells are also magnetically labeled. In addition, non-magnetically labeled cells are imaged for viability in a modified slide configuration. The labeled sample, in a chamber or cuvet, is placed between two wedge-shaped magnets to selectively move the magnetically labeled cells to the observation surface of the cuvet. An LED illuminates the cells and a CCD camera captures the images of the fluorescent light emitted by the target cells. Image analysis performed with a novel algorithm provides a count of the cells on the surface that can be related to the target cell concentration of the original sample. The compact cytometer system provides a rugged, affordable and easy-to-use technique, which can be used in remote locations.
Owner:VERIDEX LCC

Sleeve type bubbling-humidifying seawater desalinating device

The invention discloses a sleeve type bubbling-humidifying seawater desalinating device. The device comprises a solar heat collector, a bubbling evaporation cylinder, bubblers, a condensing cylinder, hot pipes, corrugated heat conducting plates, a seawater cooling cylinder, a fresh-water tank, a concentrated seawater heat exchange slot, a solar photovoltaic panel, a storage battery, an air pump, a water pump and an automatic water changing system, wherein the bubbling evaporation cylinder, the condensing cylinder and the seawater cooling cylinder form a sleeve structure from inside to outside; the solar heat collector is connected to the bubbling evaporation cylinder through a pipeline; the hot pipes and the corrugated heat conducting plates are arranged on the wall of the condensing cylinder; and the bubblers are mounted at the bottom of the bubbling evaporation cylinder. According to the sleeve type bubbling-humidifying seawater desalinating device, solar energy is utilized for supplying heat and power, and bubbling humidification and dehumidification principles are adopted, so that the device is compact in structure, high in energy utilization rate, can be used for acquiring fresh water from seawater and has very important significance to the fresh water production for fresh water resource poor regions such as islands.
Owner:SOUTH CHINA UNIV OF TECH

Zalcitabine (Ddc) Boosted Lamivudine (3Tc) Compositions for Antiretroviral Therapy

ActiveUS20080214590A1High activityImprovement of TC antiretroviral activityOrganic active ingredientsBiocideTolerabilitySide effect
Boosted cytidine analogue reverse transcriptase inhibitor antiretroviral compound is a new therapeutic anti HIV option, in combination with another drug such as a NRTI or a protease inhibitor. It's heightened and sustained antiretroviral potency is due to the increased intracellular level of 3TC triphosphate, the active form of 3TC. This effect is obtained by combining 3TC, in usual doses, with a reduced dose of ddC, in the same pharmaceutical formulation. The product could be administered twice or even once daily, which is convenient, and does not increase the pill burden for the patient. The reduced ddC dosage prevents the occurrence of ddC related side effects. Other cytidine derivatives (racemic or negative enantiomers) could have the same effects as ddC and could probably be combined with 3TC, and have the same effect. On the other hand, low dose ddC may also increase the intracellular levels of other cytidine derivatives as it does for 3TC. Boosted cytidine analogue reverse transcriptase inhibitor antiretroviral compound could also be formulated in combination with another drug such as another NRTI (e.g. abacavir) or any protease inhibitor in the same capsule or tablet. This approach offers a dual anti-HIV therapy that is as efficacious as the routine triple therapy. In this way the HIV treatment cost could be significantly reduced which is imperative for resource-poor settings. This new formulation is convenient and well tolerated with no additional toxicity than that of the combining drug (NRTI or protease inhibitor) and 3TC. Moreover, this will enable a larger number of patients to benefit from the already known 3TC effects. It will also increase the 3TC effects in those organs or HIV sanctuaries with usually reduced 3TC concentrations or activity. It could be indicated in both the initial as well as in salvage HIV therapy. It could also be used for therapy optimization or simplification. Moreover, in combination with another NRTI such as abacavir, or even alone, it could be beneficial for reducing the HIV harm in resource-poor settings.
Owner:TOMA EMIL
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