The invention discloses an automatic titration device for anaerobic fermentation buffer ability, which relates to the technical field of biological fermentation and is used for solving the problem of failure in simultaneous accurate measurement of mass concentration of total inorganic carbon (TIC) and volatile fatty acids (VFA) in anaerobic fermentation broth in the prior art. The automatic titration device comprises a power module, a man-machine exchange module, a data acquisition module, a driving transmission module and a liquid sucking/dropping module. The automatic titration device adopts the combined and integrated structure, application software provides a user operation panel, the panel of corresponding actual instrument realizes control and signal expression function of the instrument, the automatic titration device can simultaneously realize accurate measurement of mass concentration of TIC and VFA in the anaerobic fermentation broth by once titration, also can realize the functions of automatic calibration of a pH meter, automatic titration and liquid supplement, real-time generation of titration curve, data processing, display and storage, is suitable for pH, TIC and VFA mass concentration determination of anaerobic fermentation broth, and also can be used for titration of other samples.