The present invention provides a method for extracting total flavonoids from
white mulberry bark. First,
white mulberry bark is dispersed in a specific
ionic liquid solution, then dispersed in an
alcohol solution, and finally subjected to
pulse electric field treatment under specific conditions. The present invention pre-treats
white mulberry bark with a specific
ionic liquid solution, which can not only better dissolve lignocellulose and
expose the
cell membrane
protoplasm layer, but also avoid excessive
cell fragmentation, greatly reduce the exudation of
cell contents, eliminate or almost eliminate the loss of flavonoids at this stage, and significantly improve the extraction rate, purity and
antioxidant properties of total flavonoids from white mulberry bark; then,
cell membrane electroporation is achieved through specific control of the
pulse electric field treatment method and conditions, which facilitates the rapid infiltration of the
alcohol solution into the cell, and realizes the directional extraction of total flavonoids from white mulberry bark. Therefore, the present invention adopts a method of
ionic liquid solution in coordination with
pulse electric field-assisted
solvent extraction, which significantly improves the extraction rate, purity and
antioxidant properties of total flavonoids from white mulberry bark.