The invention provides a road marking reflective bead comprising titanium dioxide, calcium oxide, zirconium oxide, boron oxide, aluminum oxide and an inorganic salt additive, a preparation process of the road marking reflective bead comprises the following steps: step 1, using a sand mill for grinding and high speed dispersion of the titanium dioxide, calcium oxide, zirconium oxide, boron oxide, aluminum oxide and inorganic salt additive to obtain inorganic slurry; step 2, granulating by spray drying of the inorganic slurry; step 3, sintering the dried particles in the high-temperature oxygen-enriched environment into fine grains; step 4, cooling, collecting and screening the fine grains to obtain the road marking reflective bead. By improvement of product components, the refraction rate of crystallite ceramic beads can reach above 2.2, and the weather-proof, wear-resisting, water-tolerant and alkali resistant functions of the product and coating adhesion can be improved, so that the product has high hardness and high wear resistance, the all-weather reflective effect of the product can be ensured, and the consistency of the service life of the ceramic beads and a marking can be ensured.