The invention relates to the technical field of intelligent logistics, and provides a real-time port monitoring system based on digital twin and a working method thereof. The system comprises a port logistics system, a monitoring system, a control system, a virtual port logistics system and an operation planning system. The real-time port monitoring system provided by the invention integrates thevirtual port logistics system of a port, a physical object of the port, the monitoring system of the port and the control system of the port, thereby being able to monitor and adjust the operation process and equipment of each link of the port, and the life cycle of the logistics links of the entire port is recorded in a digital form through simulation integration. Therefore, the port can not onlyunderstand the actual operation of each link in real time, but also can perform dynamic control, speed up the innovation of the operation mode, optimize the port operation process, improve the shortcomings, and make the connection of the operation links of the entire port be higher, and the entire operation work is higher.