The invention discloses a load-membrane blowing bottle, which comprises a blowing bottle body, the inner part of the blowing bottle body is in a cavity structure, the blowing bottle is connected to a guiding circle ring, a blowing rod capable of passing through the guiding circle ring is spliced with the blowing bottle body, a blowing push plug is arranged at one end of the blowing rod, the blowing push plug is arranged in the blowing bottle body, a push block is arranged at the other end of the blowing rod, a blowing pore is arranged at the end face of the blowing bottle body, a loading groove is arranged at the end face of the blowing bottle body, the blowing pore is arranged in the loading groove, a first auxiliary pore is arranged at the end face of the blowing bottle body, the membrane is arranged at the first auxiliary pore, a second auxiliary pore is arranged at the side surface of the blowing bottle body, the membrane is arranged at the second auxiliary pore, and the blowing pore, the first auxiliary pore and the second auxiliary pore are respectively communicated to the inner part of the blowing bottle body. According to the invention, the load-membrane cultured with microbe is placed in the loading groove for providing gas source to the load-membrane and blowing the load-membrane, so that the microbe movement status in the load-membrane can be observed by a microscope.