The invention relates to a method for preparing multi-channel hollow fiber nano-filtration membranes. The method includes steps of (1), adding polymers, organic solvents and additives into a reaction kettle according to certain proportions, stirring the polymers, the organic solvents and the additives to form uniform and stable membrane casting solution, defoaming the membrane casting solution under a vacuum condition, ejecting the membrane casting solution via multi-channel hollow fiber molds under the driving effects of air pressures, feeding the membrane casting solution into external coagulating bath under certain core solution flow rate and air spacing conditions, carrying out phase transformation to form multi-channel hollow fiber basement membranes, sufficiently soaking the multi-channel hollow fiber basement membranes, then taking out the multi-channel hollow fiber basement membranes, airing the multi-channel hollow fiber basement membranes at certain temperatures, packaging the multi-channel hollow fiber basement membranes in stainless steel assemblies, plugging two ends of each stainless steel assembly by the aid of cotton and sealing the stainless steel assemblies by glue; (2), preparing aqueous-phase solution with certain concentration and oil-phase solution with certain concentration, injecting the aqueous-phase solution in multi-channel inner holes, reserving the aqueous-phase solution in the multi-channel inner holes for a certain period of time, purging the aqueous-phase solution by the aid of strong airflow, injecting the oil-phase solution into the multi-channel inner holes, reserving the oil-phase solution in the multi-channel inner holes for a certain period of time, purging the oil-phase solution by little airflow, arranging the multi-channel inner holes in an oven, carrying out reaction and taking the multi-channel inner holes out of the oven to obtain the multi-channel hollow fiber nano-filtration membranes. The method for preparing the multi-channel hollow fiber nano-filtration membranes has the advantages of high toughness and strength.