The invention relates to the technical field of high-strength wear-resistant steel plate preparation, in particular to an on-line quenched HB400-grade wear-resistant steel plate and a preparation method thereof. The steel plate comprises the following components of, in percentage by weight, 0.15%-0.18% of C, 0.30%-0.60% of Si, 0.22%-0.40% of Mn, less than or equal to 0.010% of P, less than or equal to 0.003% of S, 0.025%-0.050% of Alt, 0.10%-0.25% of Ni, 0.80%-1.00% of Cr, 0.15%-0.30% of Mo, 0.010%-0.030% of Ti, less than or equal to 0.0050% of N, less than or equal to 0.00015% of H, the balance Fe and trace impurities. The thickness of the steel plate is 8-20 mm. According to the preparation method, the manufactured wear-resistant steel plate is characterized in that the hardness value ofthe wear-resistant steel plate is 370 HBW-430 HBW, the tensile strength is 1250-1430 MPa, the elongation percentage (A50) ranges from 15% to 18%, the impact toughness value at 20 below zero is greater than or equal to 50J, excellent wear resistance is provided, meanwhile, excellent low-temperature impact toughness is provided, and wear-resistant steel plate is suitable for being used under severeworking conditions such as mining machinery and the like. In addition, different from the conventional off-line conditioning process, the wear-resistant steel plate manufactured by the method is produced by adopting an online quenching and off-line tempering process, the remarkable advantages of short production process and low manufacturing cost is realized.