The invention discloses a standard sample of imperfect corn kernels and a preparation method thereof. The standard sample of imperfect corn kernels is made from insect-eaten kernels, disease-spotted kernels, broken kernels, sprouted kernels, moldy kernels and heat-damaged kernels, which are embedded in organic resin. The invention adopts organic resin embedding technology to make standard samples of imperfect corn grains, which have excellent properties such as flame retardancy, aging resistance, non-toxicity, oxidation resistance, heat resistance, insulation, etc., and the standard samples of imperfect corn grains are developed in this inspection field. Quality control activities provide a reliable and scientific material basis, which is conducive to laboratory quality control, is conducive to improving the quality management level of the laboratory, and reduces the risk of the accuracy of laboratory test results.