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79results about How to "Low production efficiency" patented technology

Automatic terminal fitting device for a four-pin electrical connector

The present invention relates to an automatic terminal fitting device for four-pin electrical connector which may comprise a rack, power control box and conveying device provided on the rack. A rubber shell vibration disc, a terminal fitting mechanism, and a back cover vibration disc are provided on the rack along the transporting direction. The rubber shell vibration disc is connected to the front end of the conveying device by a rubber shell transportation rail. The terminal fitting mechanism is provided on the back side of the conveying device and engages with the terminal feeding device provided on the rack. The back cover vibration disc is provided on the back side of the conveying device, to the right side of the terminal fitting mechanism. And the terminal fitting mechanism may include four sets of terminal fitting devices sequentially provided on the back side of the conveying device. On the right side of the terminal fitting devices, a terminal strip bending device engaging with the conveying device is provided. The present invention effectively addresses the problems existing when fitting terminals manually during assembly, such as low production efficiency, high manpower consumption, intensive labor investment, relatively high potential safety hazard occurrence, and unsatisfactory connector defect rate.
Owner:DONGGUAN UNIV OF TECH

Method of producing short hairpin library

Described herein is a method of cloning synthetic oligos (including in situ synthesized oligos) into an (one or more) expression vector for library (e.g., shRNA library) production. The oligos are synthesized with one portion of the first stem of the hairpin, followed by a first loop sequence, the complete second stem, a second loop sequence, and finished with the remaining portion of the first stem of the hairpin. The two portions of the first stem anneal to the second stem, juxtaposing the 5′ end close to the 3′ end of the oligo. The methods described herein selected for hairpins with perfectly base-paired stems. After annealing, a ligase is added to the annealed oligos and the base-paired hairpins are preferentially annealed, and ligated, creating closed circular oligos. The now circularized hairpins served as templates for rolling circle amplification using a polymerase with high processivity. One or more primers complementary to the two strands of the amplified double stranded circular hairpins initiate the rolling circle amplification in the presence of a polymerase. Using primers (e.g., a sense and antisense primer), the rolling circle amplification yields double stranded hairpin sequences. These can be digested (e.g., using restriction enzymes) to produce a double-stranded hairpin fragment encoding a single hairpin. The fragment can be cloned into an appropriately digested vector for a variety of uses including expression.
Owner:DANA FARBER CANCER INST INC +1
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