The invention relates to a method for split packaging of the packing specification of 1 L or below of a n-butyllithium solution. The method is used for split packaging of the n-butyllithium solution in small packages, the split packaging process is carried out in an all-inert gas environment and is safe and controllable, and split-packaged products are clear and free of impurities. The method comprises the following two steps that 1, split packaging is carried out on the n-butyllithium solution, specifically, 1-1, inert gas is introduced in a suppressed mode from a gas phase (gas phase valve)of a 100 L steel cylinder containing the n-butyllithium solution, liquid flows out through the liquid phase valve, is filtered and then is connected to a glove box, split packaging is carried out in the glove box filled with the inert gas, the n-butyllithium solution is transferred into reagent bottles by virtue of pipeline transfer, and 1-2, after split packaging is completed, the reagent bottlescan be safely taken out from the glove box; and 2, n-butyllithium in a pipeline is treated, specifically, after split packaging is completed, the gas phase valve of the steel cylinder is opened for pressure relief, a split packaging valve which makes contact with the reagent bottles in the glove box are closed, argon is introduced from the right side, and the residual n-butyllithium solution in the pipeline is blown into the steel cylinder.