In order to overcome the defects of the prior art, the invention provides a real silk wool composite filament which comprises a wool yarn as a central core. More than eight cocoon filaments are adhered onto the outer side wall of the wool yarn. The content of the cocoon filaments is 25 to 40 percent. The real silk wool composite filament is prepared through a method comprising the following stepsof S1, selecting qualified cocoon materials; S2, stripping and selecting cocoons; S3, beating and picking ripe cocoons, extracting ripe cocoon filament heads, and adhering more than two ripe cocoon filament heads onto the wool yarn; S4, rotating the cocoon filaments adhered onto the wool yarn; twisting and sheathing the adhered filament threads; S5, inspecting the thickness of the twisted and sheathed filament threads; S6, drying the inspected filament threads for two times, spooling the dried filament threads onto a filament cylinder, and cutting to remove rough burrs during the spooling process. The real silk wool composite filament provided by the invention has the characteristics of wools and real silks, the occurrence of wind strands is avoided, and the later-period weaving cost is saved.