The invention discloses a multi-channel liquid detection and observation device for fluid observation, the device comprises an observation device and a detection device, the right side of the observation device is provided with the detection device, the top of the observation device is provided with a water tank body, the top of the water tank body is fixedly provided with a funnel, and the bottom of the funnel is provided with a liquid inlet pipe; a sealing plate is arranged at the top of the liquid inlet pipe, a buffer pipe is arranged in the observation device, a liquid outlet pipe is connected to the bottom of the buffer pipe, an adjusting valve is arranged at the joint of the buffer pipe and the liquid outlet pipe, the adjusting valve is composed of a movable rod, a sealing ball, a water leakage hole and a rotary buckle, and a liquid collecting groove is formed in the bottom of the observation device. According to the multi-channel liquid detection and observation device for fluid observation, through the S-shaped buffer pipe, when fluid passes through, the speed is decreased, an outlet in the bottom of the buffer pipe is sealed through the adjusting valve, the liquid is retained in the buffer pipe, or the size of the outlet is adjusted, so that the liquid slowly flows out, and observation can be conducted at any time.