The invention discloses honeycomb vinegar and a preparation method thereof and relates to a vinegar seasoning product. The vinegar comprises the following components in parts by weight: 0-8 parts of myotonin, 0-8 parts of black rice, 0-2 parts of red dates and 2-12 parts of honeycomb. The honeycomb vinegar is prepared by an acetic fermentation method of liquid backflow or solid fermentation. In the invention, the honeycomb, the myotonin, the black rice and the dates are used as raw materials, thus the vinegar has effective active substances of the honeycomb, the myotonin, the black rice and the dates. The product has good curative effect in the aspects of improving the immunity and treating the bronchitis, the rhinitis, the scirrhus, pain subcutaneous ulcer pyogenic infections, the benignprostatic hyperplasia, the arthritis, the hepatitis B, obstetrical and gynecological diseases, the impotence, the enuresis, the bone tuberculosis, stomach diseases, the pertussis and foot ringworms and has no toxic and side effects. Compared with the traditional table vinegar, the honeycomb vinegar has the advantages of gentle vinegar smell, pleasant fragrance and clear brownish red color. With the honeycomb vinegar, people can enjoy delicious food and also achieve the effect of medical health care.